Get access

Expression of Kisspeptin and its Receptor GPR54 in the First Trimester Trophoblast of Women with Recurrent Pregnancy Loss

Authors

  • Dong-Wook Park,

    1. Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women’s Healthcare Center, Kwandong University, College of Medicine, Seoul, Korea
    Search for more papers by this author
  • Sung-Ki Lee,

    1. Department of Obstetrics and Gynecology, College of Medicine, Konyang University, Daejeon, Korea
    Search for more papers by this author
  • Sung Ran Hong,

    1. Department of Pathology, Cheil General Hospital & Women’s Healthcare Center, Kwandong University, College of Medicine, Seoul, Korea
    Search for more papers by this author
  • Ae-Ra Han,

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Joanne Kwak-Kim,

    1. Reproductive Medicine, Department of Obstetrics and Gynecology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    2. Department of Microbiology and Immunology, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA
    Search for more papers by this author
  • Kwang Moon Yang

    1. Department of Obstetrics and Gynecology, Cheil General Hospital & Women’s Healthcare Center, Kwandong University, College of Medicine, Seoul, Korea
    Search for more papers by this author

Kwang Moon Yang, Department of Obstetrics and Gynecology, Cheil General Hospital and Women’s Healthcare Center, Kwandong University, College of Medicine, 1-19 Mookjung-Dong, Jung-Gu, Seoul 100-380, Korea.
E-mail: km1yang@naver.com

Abstract

Citation Park D-W, Lee S-K, Hong SR, Han A-R, Kwak-Kim J, Yang KM. Expression of kisspeptin and its receptor GPR54 in the first trimester trophoblast of women with recurrent pregnancy loss (RPL). Am J Reprod Immunol 2012; 67: 132–139

Problem  Kisspeptin and its receptor GPR54 play a major role in trophoblast invasion. The expression of kisspeptin and GPR54 in trophoblast and decidua and their relationship with decidual and peripheral blood natural killer (NK) cells are investigated in women with RPL.

Method of study  Trophoblast and decidual tissues were collected from 38 RPL women who miscarried a genetically normal fetus and 14 women who had elective abortion. Kisspeptin, GPR54, and decidual NK cells were investigated with immunohistochemistry, and peripheral blood NK cells were analyzed by flow cytometry.

Results  Kisspeptin expression in syncytiotrophoblast was significantly decreased in RPL women with normal (<15%) peripheral blood NK cells (npNK) (P = 0.021) and high (≥15%) peripheral blood NK cells (hpNK) (P = 0.024) as compared to controls. Kisspeptin expression in cytotrophoblast was significantly decreased hpNK group (P = 0.009) as compared to controls. GPR54 expressions were not different among study groups and controls. The number of CD56+ decidual NK cells are significantly higher in hpNK group as compared to npNK group (P = 0.041) and showed a correlation with kisspeptin expression in syncytiotrophoblasts (r = 0.738, P < 0.001).

Conclusion  Decreased kisspeptin expression in trophoblasts is associated with RPL and kisspeptin may engage the regulation of decidual NK cell infiltration.

Ancillary