Microparticles of Pregnant Women and Preeclamptic Patients Activate Endothelial Cells in the Presence of Monocytes
Version of Record online: 20 OCT 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 3, pages 206–215, March 2012
How to Cite
Lok, C. A. R., Snijder, K. S., Nieuwland, R., Van Der Post, J. A. M., de Vos, P. and Faas, M. M. (2012), Microparticles of Pregnant Women and Preeclamptic Patients Activate Endothelial Cells in the Presence of Monocytes. American Journal of Reproductive Immunology, 67: 206–215. doi: 10.1111/j.1600-0897.2011.01079.x
- Issue online: 29 JAN 2012
- Version of Record online: 20 OCT 2011
- Submitted July 21, 2011; accepted September 21, 2011.
- Endothelial cells;
Citation Lok CAR, Snijder KS, Nieuwland R, Van Der Post JAM, de Vos P, Faas MM. Microparticles of pregnant women and preeclamptic patients activate endothelial cells in the presence of monocytes. Am J Reprod Immunol 2012; 67: 206–215
Problem Preeclampsia is a pregnancy-specific disorder that may result from an adverse maternal response to circulating placenta-derived factors, causing a systemic inflammation including endothelial activation. Plasma from preeclamptic patients was shown to induce endothelial activation in the presence of monocytes. We investigated whether microparticles (MP) are the plasma factors causing this activation of endothelial cells.
Method of study Monocultures and co-cultures of monocytes and endothelial cells were incubated with plasma, MP-poor plasma or isolated MP from non-pregnant and pregnant women and preeclamptic patients (each n = 8). ICAM-1 expression was analyzed with flow cytometry.
Results The expression of ICAM-1 was significantly increased in monocytes and endothelial cells in co-cultures after the addition of isolated MP from preeclamptic patients (P = 0.017) and to a lesser extent in pregnant women (P = 0.012) compared to non-pregnant controls.
Conclusions Microparticles from preeclamptic patients activate endothelial cells in the presence of monocytes. Whether all MP have the same effect on monocytes and endothelial cells or only a specific subgroup is the focus of future research.