Identification of Immune Cells by Flow Cytometry in Vaginal Lavages from Women with Vulvovaginitis and Normal Microflora
Version of Record online: 8 DEC 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 3, pages 198–205, March 2012
How to Cite
Giraldo, P. C., de Carvalho, J. B. J., do Amaral, R. L. G., da Silveira Gonçalves, A. K., Eleutério, J. and Guimarães, F. (2012), Identification of Immune Cells by Flow Cytometry in Vaginal Lavages from Women with Vulvovaginitis and Normal Microflora. American Journal of Reproductive Immunology, 67: 198–205. doi: 10.1111/j.1600-0897.2011.01093.x
- Issue online: 29 JAN 2012
- Version of Record online: 8 DEC 2011
- Submitted July 27, 2011; accepted October 20, 2011.
- Bacterial vaginosis;
- flow cytometry;
- vulvovaginal candidiasis
Citation Giraldo PC, de Carvalho JBJ, do Amaral RLG, da Silveira Gonçalves AK, Eleutério J Jr, Guimarães F. Identification of Immune Cells by Flow Cytometry in Vaginal Lavages from Women with Vulvovaginitis and Normal Microflora. Am J Reprod Immunol 2012; 67: 198–205
Problem The extent of the vaginal immune response is not fully determined. The purpose of this study was to evaluate the vaginal immune cells from women with vulvovaginitis (VV).
Method of Study A total of 142 volunteers diagnosed with bacterial vaginosis (BV), vulvovaginal candidiasis (VC), and BV associated with VC or normal microflora were sampled to evaluate the immune cells by flow cytometry. The immune cells were obtained by vaginal lavage and labeled with fluorochrome-conjugated monoclonal antibodies to identify neutrophil granulocytes, macrophages, CD4+ and CD8+ T lymphocytes, B lymphocytes, and NK lymphocytes.
Results Neutrophil granulocytes were present in 84.6% of samples among the leukocyte populations. Considering samples in which neutrophils were present, the mean percentage of neutrophil granulocytes was significantly higher in women with VC than BV and normal microflora and was significantly lower in women with BV than normal microflora. Macrophages and lymphocytes were present in a lower percentage of samples. The mean percentage of CD4+ T lymphocytes in vaginal lavages was significantly higher in VC and BV compared with women with normal microflora.
Conclusions Neutrophils were the predominant leukocytes and were associated with VC and inversely with BV. CD4+ T lymphocytes were associated with both VC and BV.