An In Vitro Model for the Study of Human Implantation
Article first published online: 12 DEC 2011
© 2011 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 2, pages 169–178, February 2012
How to Cite
Holmberg, J. C., Haddad, S., Wünsche, V., Yang, Y., Aldo, P. B., Gnainsky, Y., Granot, I., Dekel, N. and Mor, G. (2012), An In Vitro Model for the Study of Human Implantation. American Journal of Reproductive Immunology, 67: 169–178. doi: 10.1111/j.1600-0897.2011.01095.x
- Issue published online: 4 JAN 2012
- Article first published online: 12 DEC 2011
- Submitted October 24, 2011; accepted November 1, 2011.
Citation Holmberg JC, Haddad S, Wünsche V, Yang Y, Aldo PB, Gnainsky Y, Granot I, Dekel N, Mor G. An In vitro model for the study of human implantation. Am J Reprod Immunol 2012; 67: 169–178
Problem Implantation remains the rate-limiting step for the success of in vitro fertilization. Appropriate models to study the molecular aspects of human implantation are necessary in order to improve fertility.
Methods First trimester trophoblast cells are differentiated into blastocyst-like spheroids (BLS) by culturing them in low attachment plates. Immortalized human endometrial stromal cells and epithelial cells (ECC-1) were stably transfected with GFP or tdTomato. Co-culture experiments were monitored using Volocity imaging analysis system.
Results This method demonstrates attachment and invasion of BLS, formed by trophoblast cells, into stromal cells, but not to uterine epithelial cells.
Conclusion We have developed an in vitro model of uterine implantation. The manipulation of this system allows for dual color monitoring of the cells over time. Additionally, specific compounds can be added to the culture media to test how this may affect implantation and invasion. This model is a helpful tool in understanding the complexity of human implantation.