Placental Villous Expression of TNFα and IL-10 and Effect of Oxygen Tension in Euploid Early Pregnancy Failure
Article first published online: 16 JAN 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 6, pages 515–525, June 2012
How to Cite
Calleja-Agius J, Jauniaux E, Muttukrishna S. Placental villous expression of TNFα and IL-10 and effect of oxygen tension in euploid early pregnancy failure. Am J Reprod Immunol 2012; 67: 515–525
- Issue published online: 10 MAY 2012
- Article first published online: 16 JAN 2012
- Manuscript Accepted: 20 DEC 2011
- Manuscript Received: 22 NOV 2011
- Interleukin 10;
- TNF-receptor 1;
- TNF-receptor 2;
The objective was to investigate placental inflammation in chromosomally normal miscarriages in vivo and in vitro.
Method of study
Chorionic villous tissue was collected from missed miscarriages and normal gestation-matched controls and cultured at 6 and 20% O2 concentrations. Tissue was karyotyped. Flowcytometric bead arrays and real-time PCR were carried out for protein and gene expression studies.
The levels of TNFα and IL-10 were significantly (P < 0.005 and P < 0.05) higher, and the levels of TNF-R1 and TNF-R2 were significantly (P < 0.01 and P < 0.05) lower in culture conditioned medium of villous explants of miscarriages compared to control group. Villous tissue homogenates from miscarriages contained significantly (P < 0.005) lower levels of TNF-R1. There was a significant O2-dependent increase in the secretion of IL-10 (P < 0.01) and decrease in TNFα/IL-10 ratio (P < 0.005) in the culture medium in both groups.
Increased levels of TNFα and decreased levels of receptors in miscarriage villous tissue confirm an excessive placental inflammation in miscarriage patients.