Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
Article first published online: 13 FEB 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 67, Issue 6, pages 526–539, June 2012
How to Cite
Borges AM, Healey GD, Sheldon IM. Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo. Am J Reprod Immunol 2012; 67: 526–539
- Issue published online: 10 MAY 2012
- Article first published online: 13 FEB 2012
- Manuscript Accepted: 15 JAN 2012
- Manuscript Received: 10 OCT 2011
- Conselho Nacional de Desenvolvimento Científico e Tecnológico. Grant Number: PDE 200885/2010-3
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais. Grant Number: APQ 02489-10
- UK Biotechnology and Biological Sciences Research Council. Grant Number: BB/I017240/1
- innate immunity
Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation.
Method of study
Intact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin–dUTP nick-end labelling and lactate dehydrogenase assay.
Intact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium.
This study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.