The Mucin MUC16 (CA125) Binds to NK Cells and Monocytes from Peripheral Blood of Women with Healthy Pregnancy and Preeclampsia
Article first published online: 1 MAR 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 68, Issue 1, pages 28–37, July 2012
How to Cite
- Issue published online: 5 JUN 2012
- Article first published online: 1 MAR 2012
- Manuscript Accepted: 30 JAN 2012
- Manuscript Received: 7 DEC 2011
- National Institutes of Health. Grant Number: R21CA143616
- Wisconsin Comprehensive Cancer Centers Flow Cytometry. Grant Number: CA14520
- immune cell subsets;
MUC16 (CA125) released from ovarian tumors binds to NK cells and monocytes via the inhibitory receptor Siglec-9. Here, we investigate whether MUC16 also binds to circulating immune cells during pregnancy and in women with preeclampsia.
Method of study
MUC16 binding was monitored by flow cytometry and immunoprecipitation, and RT-PCR was used to monitor indigenous expression in immune cells. Serum CA125 levels were measured by a clinical assay.
MUC16 was equally distributed on Siglec-9pos CD16pos/CD56dim and CD16neg/CD56br NK cells in the healthy pregnant and preeclampsia groups. While serum CA125 levels and number of NK and monocytes were similar, increased binding of MUC16 was observed on these immune cells in the preeclampsia cohort as compared to the healthy pregnant samples.
MUC16 binding to NK cells and monocytes likely contributes to tolerance of the fetal allograft from maternal responses and may also serve as a novel biomarker for preeclampsia.