The Association Between Endometriosis and Polymorphisms in the Interleukin-1 Family Genes in Korean Women

Authors

  • Sungwook Chun,

    1. Department of Obstetrics and Gynecology, College of Medicine, Inje University, Haeundae Paik Hospital, Busan, Korea
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  • Hoon Kim,

    1. Department of Obstetrics and Gynecology, Incheon Medical Center, Incheon, Korea
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  • Seung-Yup Ku,

    1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea
    2. Clinical Research Institute, Seoul National University, Seoul, Korea
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  • Chang Suk Suh,

    1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea
    2. Clinical Research Institute, Seoul National University, Seoul, Korea
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  • Seok Hyun Kim,

    1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea
    2. Clinical Research Institute, Seoul National University, Seoul, Korea
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  • Jung Gu Kim

    Corresponding author
    1. Clinical Research Institute, Seoul National University, Seoul, Korea
    • Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea
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Correspondence

Jung Gu Kim, Department of Obstetrics and Gynecology, Seoul National University Hospital, 28 Yeungun-dong, Chongno-Ku, Seoul 110-744, Korea.

E-mail: kimjg@plaza.snu.ac.kr

Abstract

Problem

To investigate the association between polymorphisms of the interleukin-1 (IL-1) family genes and endometriosis in Korean women.

Method of study

In this case–control study, the IL-1α −889C>T, IL-1 receptor antagonist (IL-1RA) 86-bp microsatellite, IL-1 receptor 1 (IL-1R1) 52C>A, 294C>T, 1498T>C, 1632A>G,IL-1R2 rs2072472 C>T and rs7561460 C>T polymorphisms were analyzed in women with (n = 138) and without (n = 214) endometriosis using restriction fragment length polymorphism (RFLP) analysis, TaqMan assay, or DNA sequencing. Serum IL-1α, soluble IL-1RA (sIL-1RA), and sIL-1R2 levels were measured by enzyme-linked immunosorbent assay (ELISA).

Results

Among the polymorphisms measured, the 1498T>C polymorphisms in the IL-1R1 gene were found to be related with early-stage endometriosis but not with advanced-stage endometriosis. The genotypes with at least one T allele (CT + TT) were less frequently observed in early-stage endometriosis compared with normal controls (OR = 0.44, 95% CI = 0.22–0.87, P = 0.02). Serum sIL-1R2 levels were significantly lower (< 0.01) in women with endometriosis than in normal controls, whereas no difference in serum sIL-1RA levels between these two groups was noted. The single and haplotype genotypes of the IL-1R2 and IL-1RA microsatellite polymorphisms were not related with these serum levels.

Conclusion

The IL-1R1 1498T>C polymorphism is associated with early-stage endometriosis in Korean women.

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