Comprehensive Analysis of Peripheral Blood Lymphocytes in 76 Women with Recurrent Miscarriage before and after Lymphocyte Immunotherapy

Authors

  • Peiyan Liang,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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    • P. Liang and M. Mo contributed equally to this work.
  • Meilan Mo,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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    • P. Liang and M. Mo contributed equally to this work.
  • Guan-Gui Li,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Biao Yin,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Jing Cai,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Tonghua Wu,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Xuebing He,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Xu Zhang,

    1. Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    3. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
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  • Yong Zeng

    Corresponding author
    1. Fertility Center, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
    2. Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen, China
    • Clinical Center for Recurrent Spontaneous Abortion & Recurrent Implantation Failure, Shenzhen Zhongshan Urological Hospital, Shenzhen, China
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Correspondence

Yong Zeng, Fertility Center, Shenzhen Zhongshan Urology Hospital, No. 1001 Fuqiang Road, Futian District, Shenzhen, 518045, China.

E-mail: szsz01@sina.com

Abstract

Problem

The efficacy of lymphocyte immunotherapy (LIT) for unexplained recurrent miscarriage (uRM) remains indefinite. The objective of the present study is to comprehensively evaluate the alterations in the proportions and functions of peripheral blood lymphocytes in patients with uRM before and after LIT.

Method of study

Seventy-six women with uRM were included in the present study. Immunophenotype of peripheral blood lymphocytes (76 patients), NK cytotoxicity (62 patients) and Th1 (IFN-γ and TNF-α)/Th2 (IL-10) ratios (73 patients) were assessed during the luteal phase before LIT and after the third LIT by the flow cytometer.

Results

To date, 29 patients had already delivered or been pregnant more than 20 weeks (successful pregnancy group) and 5 patients experienced subsequent abortion (abortion group). The percentages of CD3+ T cell, CD3+ CD4+ T cell and IL-10-producing Th1 cell were significantly increased, whereas the percentages of CD3+ HLA-DR+ T cell, CD56+ CD3 NK cell and CD56dim CD16+ NK cell, the NK cytotoxicity and the ratios of Th1/Th2 were significantly decreased after LIT in the total patients and in the successful pregnant group. The percentages of CD56CD3 NK cell and IFN-γ-producing cell, and the ratio of IFN-γ/IL-10 were significantly lower after LIT in the successful pregnant group compared to those in the abortion group.

Conclusions

LIT alters the proportions and functions of most peripheral blood lymphocyte subsets. Some of these alterations may be beneficial for pregnancy maintenance, whereas some may be potential markers for predicting subsequent abortion.

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