Chunhua Yu, Li Wang and Jianhua Li contributed equally to this article.
Ovarian Stimulation Reduces IL-6 Release from Mouse and Human Pre-Implantation Embryos
Article first published online: 10 MAY 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 68, Issue 3, pages 199–204, September 2012
How to Cite
Yu C, Wang L, Li J, Guo C, Guo X, Zhang X, Xu Y, Yao Y. Ovarian stimulation reduces IL-6 release from mouse and human pre-implantation embryos. Am J Reprod Immunol 2012; 68: 199–204
- Issue published online: 12 AUG 2012
- Article first published online: 10 MAY 2012
- Manuscript Accepted: 21 MAR 2012
- Manuscript Received: 31 DEC 2011
- ovarian stimulation
In our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse blastocysts and IL-6 protein release to the culture medium from mouse and human pre-implantation embryos.
Method of study
IL-6 mRNA were measured by PCR and IL-6 release to the culture medium were measured by ELISA.
Superovulation caused a 1.95-fold reduction in IL-6 mRNA in mouse blastocysts and reduced IL-6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 ± 0.635 versus 4.799 ± 0.312 pg/mL). Ovarian stimulation also significantly reduced IL-6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 ± 0.945 versus 4.543 ± 0.796 pg/mL).
Our findings indicate that IL-6 might serve as a candidate marker for evaluating embryo developmental competence.