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Ovarian Stimulation Reduces IL-6 Release from Mouse and Human Pre-Implantation Embryos

Authors

  • Chunhua Yu,

    1. Department of Obstetric and Gynecology, TangDu Hospital, The Fourth Military Medical University, Xi'an, China
    2. Department of Obstetric and Gynecology, The Inner Mongolia Provincial Corps Hospital of Chinese People's Armed Police Forces, Hohhot, China
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  • Li Wang,

    1. Department of Obstetrics and Gynecology, The General Hospital of the People's Liberation Army, Beijing, China
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  • Jianhua Li,

    1. Department of Obstetrics and Gynecology, General Hospital of Beijing Military Command, Beijing, China
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  • Chun Guo,

    1. Department of Obstetric and Gynecology, The Inner Mongolia Provincial Corps Hospital of Chinese People's Armed Police Forces, Hohhot, China
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  • Xinyu Guo,

    1. Department of Obstetrics and Gynecology, The General Hospital of the People's Liberation Army, Beijing, China
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  • Xinyan Zhang,

    1. Department of Obstetrics and Gynecology, The General Hospital of the People's Liberation Army, Beijing, China
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  • Ye Xu,

    Corresponding author
    1. Department of Obstetrics and Gynecology, General Hospital of Beijing Military Command, Beijing, China
    • Department of Obstetric and Gynecology, TangDu Hospital, The Fourth Military Medical University, Xi'an, China
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  • Yuanqing Yao

    Corresponding author
    1. Department of Obstetrics and Gynecology, The General Hospital of the People's Liberation Army, Beijing, China
    • Department of Obstetric and Gynecology, TangDu Hospital, The Fourth Military Medical University, Xi'an, China
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  • Chunhua Yu, Li Wang and Jianhua Li contributed equally to this article.

Correspondence

Yuanqing Yao, Department of Obstetrics and Gynecology, The General Hospital of the People's Liberation Army, 28 Fuxing Rd, Beijing 100853, China.

E-mail: yqyaolab@gmail.com

Ye Xu, Department of Obstetrics and Gynecology, General Hospital of Beijing Military Command, 5 Nancangmen, Beijing 100700, China.

E-mail: yexu1001@sohu.com

Abstract

Problem

In our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse blastocysts and IL-6 protein release to the culture medium from mouse and human pre-implantation embryos.

Method of study

IL-6 mRNA were measured by PCR and IL-6 release to the culture medium were measured by ELISA.

Results

Superovulation caused a 1.95-fold reduction in IL-6 mRNA in mouse blastocysts and reduced IL-6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 ± 0.635 versus 4.799 ± 0.312 pg/mL). Ovarian stimulation also significantly reduced IL-6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 ± 0.945 versus 4.543 ± 0.796 pg/mL).

Conclusion

Our findings indicate that IL-6 might serve as a candidate marker for evaluating embryo developmental competence.

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