Analyses of Peripheral Blood Mononuclear Cells in Operational Tolerance After Pediatric Living Donor Liver Transplantation

Authors

  • Ying Li,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Takaaki Koshiba,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Atsushi Yoshizawa,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Yukihide Yonekawa,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Kosuke Masuda,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Atsushi Ito,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Mikiko Ueda,

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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  • Takahide Mori,

    1. Daigo Watanabe Clinic, 30–15 Takahata-cho Daigo Fushimiku, 601–1375, Kyoto, Japan
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  • Hiroshi Kawamoto,

    1. Laboratory of Immunology and Cell Biology and Laboratory for Lymphocyte Development, RIKEN RCAI, Graduate School of Biostudies, Kyoto University
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  • Yoshimasa Tanaka,

    Corresponding author
    1. Laboratory of Immunology and Cell Biology and Laboratory for Lymphocyte Development, RIKEN RCAI, Graduate School of Biostudies, Kyoto University
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  • Shimon Sakaguchi,

    1. Department of Experimental pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
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  • Nagahiro Minato,

    1. Laboratory of Immunology and Cell Biology and Laboratory for Lymphocyte Development, RIKEN RCAI, Graduate School of Biostudies, Kyoto University
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  • Kathryn J. Wood,

    1. Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Headington, Oxford, UK
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  • Koichi Tanaka

    1. Department of Transplantation and Immunology and Horizontal Medical Research Organization, Transplant Tolerance Unit, Kyoto University, Faculty of medicine, Kyoto, Japan
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*Corresponding authors: Koichi Tanaka, koichi@kuhp.kyoto-u.ac.jp, Takaaki Koshiba, tkoshiba@kuhp.kyoto-u.ac.jp

Abstract

Operational tolerance (graft acceptance in an immunosuppression (IS)-free environment) after living-donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4+CD25high+ cells, B cells and Vδ1/Vδ2 γδT-cells ratio in operationally tolerant patients (Gr-tol; n = 12), compared with those from age-matched volunteers (Gr-vol; n = 24) or patients on IS (Gr-IS; n = 19). The frequency of NK cells was decreased in Gr-tol, compared with those in Gr-IS or Gr-vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr-vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.

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