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Detection of Insulin mRNA in the Peripheral Blood after Human Islet Transplantion Predicts Deterioration of Metabolic Control

  1. T. Berney1,*,
  2. A. Mamin2,
  3. A. M. James Shapiro3,
  4. B. Ritz-Laser2,
  5. M.-C. Brulhart2,
  6. C. Toso1,
  7. S. Demuylder-Mischler1,
  8. M. Armanet1,
  9. R. Baertschiger1,
  10. A. Wojtusciszyn1,
  11. P.-Y. Benhamou4,
  12. D. Bosco1,
  13. P. Morel1,
  14. J. Philippe2

Article first published online: 17 MAY 2006

DOI: 10.1111/j.1600-6143.2006.01373.x

Issue

American Journal of Transplantation

American Journal of Transplantation

Volume 6, Issue 7, pages 1704–1711, July 2006

Additional Information

How to Cite

Berney, T., Mamin, A., James Shapiro, A. M., Ritz-Laser, B., Brulhart, M.-C., Toso, C., Demuylder-Mischler, S., Armanet, M., Baertschiger, R., Wojtusciszyn, A., Benhamou, P.-Y., Bosco, D., Morel, P. and Philippe, J. (2006), Detection of Insulin mRNA in the Peripheral Blood after Human Islet Transplantion Predicts Deterioration of Metabolic Control. American Journal of Transplantation, 6: 1704–1711. doi: 10.1111/j.1600-6143.2006.01373.x

Author Information

  1. 1

    Division of Visceral and Transplant Surgery

  2. 2

    Division of Diabetes and Endocrinology, Geneva University Hospitals, Geneva, Switzerland

  3. 3

    Department of Surgery, University of Alberta, Edmonton, Canada

  4. 4

    Division of Nephrology, Diabetes and Endocrinology, University Hospital Center, Grenoble, France

* * Corresponding author: T. Berney, thierry.berney@hcuge.ch

Publication History

  1. Issue published online: 17 MAY 2006
  2. Article first published online: 17 MAY 2006
  3. Received 14 February 2006, revised 14 March 2006 and accepted for publication 30 March 2006

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References

  • 1
    Shapiro AMJ, Lakey JRT, Ryan E et al. Islet transplantation in seven patients with type 1 diabetes mellitus using steroid-free immunosuppression regimen. N Engl J Med 2000; 343: 230–238.
  • 2
    Ryan EA, Paty BW, Senior PA et al. Five-year follow-up after clinical islet transplantation. Diabetes 2005; 54: 2060–2069.
  • 3
    Molano RD, Berney T, Li H et al. Prolonged islet graft survival in NOD mice by blockade of the CD40-CD154 pathway of T-cell co-stimulation. Diabetes 2001; 50: 270–276.
  • 4
    Jaeger C, Brendel MD, Eckhard M, Bretzel RG. Islet autoantibodies as potential markers for disease recurrence in clinical islet transplantation. Exp Clin Endocrinol Diabetes 2000; 108: 328–333.
  • 5
    Ricordi C, Zeng YJ, Alejandro R et al. In vivo effects of FK506 on human pancreatic islets. Transplantation 1991; 52: 519–522.
  • 6
    Paty BW, Harmon JS, Marsh CL, Robertson RP. Inhibitory effects of immunosuppressive drugs on insulin secretion from HIT-T15 cells and Wistar rat islets. Transplantation 2002; 73: 353–357.
  • 7
    Bell E, Cao X, Moibi JA et al. Rapamycin has a deleterious effect on MIN-6 cells and rat and human islets. Diabetes 2003; 52: 2731–2739.
  • 8
    Ryan EA, Lakey JRT, Rajotte RV et al. Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol. Diabetes 2001; 50: 710–719.
  • 9
    Bottino R, Fernandez LA, Ricordi C et al. Transplantation of allogeneic islets of Langerhans in the rat liver: Effects of macrophage depletion on graft survival and microenvironment activation. Diabetes 1998; 47: 316–323.
  • 10
    Cattan P, Berney T, Schena S et al. Early assessment of apoptosis in isolated islets of Langerhans. Transplantation 2001; 71: 857–862.
  • 11
    Li X, Chen H, Epstein PN. Metallothionein protects islets from hypoxia and extends islet graft survival by scavenging most kinds of reactive oxygen species. J Biol Chem 2004; 279: 765–771.
  • 12
    Bottino R, Balamurugan AN, Tse H et al. Response of human islets to isolation stress and the effect of antioxidant treatment. Diabetes 2004; 53: 2259–2268.
  • 13
    Berney T, Molano RD, Cattan P et al. Endotoxin-mediated delayed islet graft function is associated with increased intra-islet cytokine production and islet cell apoptosis. Transplantation 2001; 71: 125–132.
  • 14
    Ritz-Laser B, Oberholzer J, Toso C et al. Molecular detection of circulating β-cells after islet transplantation. Diabetes 2002; 51: 557–561.
  • 15
    Lee TC, Barshes NR, Brunicardi FC et al. Procurement of the human pancréas for pancreatic islet isolation. Transplantation 2004; 78: 481–483.
  • 16
    Ricordi C, Lacy PE, Finke EH et al. Automated method for isolation of human pancreatic islets. Diabetes 1988; 37: 413–420.
  • 17
    Bucher P, Mathe Z, Morel P et al. Assessment of a novel two-component enzyme preparation for human islet isolation and transplantation. Transplantation 2005; 79: 91–97.
  • 18
    Berney T, Pileggi A, Molano R, Ricordi C. Pancreatic islet cells. In: AtalaA, LanzaR, eds. Methods of tissue engineering. San Diego : Academic Press, 2001: 203–218.
  • 19
    Goss JA, Soltes G, Goodpastor SE et al. Pancreatic islet transplantation: The radiographic approach. Transplantation 2003; 76: 199–203.
  • 20
    Berney T, Ricordi C. Islet transplantation: The future? Langenbecks Arch Surg 2000; 385: 373–378.
  • 21
    Wang J, Shen L, Najafi H et al. Regulation of insulin preRNA splicing by glucose. Proc Natl Acad Sci U S A 1997; 94: 4360–4365.
  • 22
    Pileggi A, Ricordi C, Alessiani M, Inverardi L. Factors influencing islet of Langerhans graft function and monitoring. Clin Chim Acta 2001; 310: 3–16.
  • 23
    Shapiro AMJ, Hao EG, Lakey JRT et al. Novel approaches toward early diagnosis of islet allograft rejection. Transplantation 2001; 71: 1709–1718.
  • 24
    Olack BJ, Swanson CJ, Flavin KS et al. Sensitization to HLA antigens in islet recipients with failing transplants. Transplant Proc 1997; 29: 2268–2269.
  • 25
    Bosi E, Braghi S, Maffi P et al. Autoantibody response to islet transplantation in type 1 diabetes. Diabetes 2001; 50: 2464–2471.
  • 26
    Roep BO, Stobbe I, Duinkerken G et al. Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. Diabetes 1999; 48: 484–490.
  • 27
    Reijonen H, Kwok WW, Nepom GT. Detection of CD4+ autoreactive T cells in T1D using HLA class II tetramers. Ann N Y Acad Sci 2003; 1005: 82–87.
    Direct Link:
  • 28
    Mallone R, Nepom GT. MHC class II tetramers and the pursuit of antigen-specific T cells: Define, deviate, delete. Clin Immunol 2003; 110: 232–242.
  • 29
    Paty BW, Bonner-Weir S, Laughlin MR, McEwan AJ, Shapiro AM. Toward development of imaging modalities for islets after transplantation: Insights from the National Institutes of Health Workshop on Beta Cell Imaging. Transplantation 2004; 77: 1133–1137.
  • 30
    Jirak D, Kriz J, Herynek V et al. MRI of transplanted pancreatic islets. Magn Reson Med 2004; 52: 1228–1233.
    Direct Link:
  • 31
    Han D, Xu X, Pastori RL, Ricordi C, Kenyon NS. Elevation of cytotoxic lymphocyte gene expression is predictive of islet allograft rejection in nonhuman primates. Diabetes 2002; 51: 562–566.
  • 32
    Han D, Xu X, Baidal D et al. Assessment of cytotoxic lymphocyte gene expression in the peripheral blood of human islet allograft recipients. Elevation precedes clinical evidence of rejection. Diabetes 2004; 53: 2281–2290.
  • 33
    Bennet W, Sundberg B, Groth CG et al. Incompatibility between human blood and isolated islets of Langerhans: A finding with implications for clinical intraportal islet transplantation? Diabetes 1999; 48: 1907–1914.
  • 34
    Davalli AM, Scaglia L, Zangen DH et al. Vulnerability of islets in the immediate posttransplantation period. Dynamic changes in structure and function. Diabetes 1996; 45: 1161–1167.
  • 35
    Menger MD, Yamauchi J, Vollmar B. Revascularization and microcirculation of freely grafted islets of Langerhans. World J Surg 2001; 25: 509–515.
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