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The ADP/ATP Ratio: A Novel Predictive Assay for Quality Assessment of Isolated Pancreatic Islets
Article first published online: 26 JUL 2006
DOI: 10.1111/j.1600-6143.2006.01474.x
Additional Information
How to Cite
Goto, M., Holgersson, J., Kumagai-Braesch, M. and Korsgren, O. (2006), The ADP/ATP Ratio: A Novel Predictive Assay for Quality Assessment of Isolated Pancreatic Islets. American Journal of Transplantation, 6: 2483–2487. doi: 10.1111/j.1600-6143.2006.01474.x
Publication History
- Issue published online: 26 JUL 2006
- Article first published online: 26 JUL 2006
- Received 14 February 2006, revised 4 June 2006 and accepted for publication 8 June 2006
- Abstract
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Keywords:
- ADP/ATP ratio;
- adult porcine islets;
- islet transplantation;
- viability
Measurement of ADP/ATP ratio in isolated pig islets predicted their ability to cure athymic diabetic mice, suggesting this may be useful for predicting performance of human islet preparations.
The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.