T. Berney and J.-P. Vallée contributed equally to this work as co-senior authors.
A Novel Method for Quantitative Monitoring of Transplanted Islets of Langerhans by Positive Contrast Magnetic Resonance Imaging
Article first published online: 12 MAY 2011
©2011 The Authors Journal compilation©2011 The American Society of Transplantation and the American Society of Transplant Surgeons
American Journal of Transplantation
Volume 11, Issue 6, pages 1158–1168, June 2011
How to Cite
Crowe, L. A., Ris, F., Nielles-Vallespin, S., Speier, P., Masson, S., Armanet, M., Morel, P., Toso, C., Bosco, D., Berney, T. and Vallee, J.-P. (2011), A Novel Method for Quantitative Monitoring of Transplanted Islets of Langerhans by Positive Contrast Magnetic Resonance Imaging. American Journal of Transplantation, 11: 1158–1168. doi: 10.1111/j.1600-6143.2011.03559.x
- Issue published online: 6 JUN 2011
- Article first published online: 12 MAY 2011
- Received 27 July 2010, revised 04 February 2011 and accepted for publication 04 March 2011
- Iron oxide;
- ultrashort TE
The Automatic Quantitative Ultrashort Echo Time imaging (AQUTE) protocol for serial MRI allows quantitative in vivo monitoring of iron labeled pancreatic islets of Langerhans transplanted into the liver, quantifying graft implantation and persistence in a rodent model. Rats (n = 14), transplanted with iron oxide loaded cells (0–4000 islet equivalents, IEQ), were imaged using a 3D radial ultrashort echo time difference technique (dUTE) on a Siemens MAGNETOM 3T clinical scanner up to 5 months postsurgery. In vivo 3D dUTE images gave positive contrast from labeled cells, suppressing liver signal and small vessels, allowing automatic quantification. Position of labeled islet clusters was consistent over time and quantification of hyperintense pixels correlated with the number of injected IEQs (R2= 0.898, p < 0.0001), and showed persistence over time (5 months posttransplantation). Automatic quantification was superior to standard imaging and manual counting methods, due to the uniform suppressed background and high contrast, resulting in significant timesavings, reproducibility and ease of quantification. Three-dimensional coverage of the whole liver in the absence of cardiac/respiratory artifact provided further improvement over conventional imaging. This imaging protocol reliably quantifies transplanted islet mass and has high translational potential to clinical studies of transplanted pancreatic islets.