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Video 1: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 1 after 8 h of cold preservation. Discontinous, sinuous ZO-1 staining (green) and very limited, sporadic colocalization (appearing as yellow staining) with claudin-3 (red). The round opening of a goblet cell is visible to the right of the image.

Video 2: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 2 after 8 h of cold preservation. Straight, less tensioned ZO-1 staining pattern (green) and ZO-1/claudin-3 colocalization present (appearing as yellow staining)

Video 3: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 3 after 8 h of cold preservation. Sinuous ZO-1 staining (green) and some colocalization present (yellow staining) with claudin-3 (red)

Video 4: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 1 after 14 h of cold preservation. Thinner and discontinuous ZO-1 staining (green), absent ZO-1/claudin-3 colocalization and diffuse claudin-3 staining pattern (red) that may be observed inside the ring (i.e. intracellular)

Video 5: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 2 after 14 h of cold preservation. Well preserved honeycomb pattern, sinuous, continuous ZO-1 staining (green) and some degree of ZO-1/claudin-3 colocalization (yellow) can be seen. Claudin-3 stains diffuse, some intracellular staining present. The oval opening of a goblet cell is visible close to the center of the image

Video 6: Tridimensional reconstruction of the perijunctional ring (en face view) in the intestines of group 3 after 14 h of cold preservation. Well preserved honeycomb pattern, sinuous, continuous and weak ZO-1 staining (green) and absent ZO-1/claudin-3 colocalization (yellow). Claudin-3 (red) stains weak.

FilenameFormatSizeDescription
AJT_4067_sm_MOVIE_1.avi305KSupporting info item
AJT_4067_sm_MOVIE_2.avi265KSupporting info item
AJT_4067_sm_MOVIE_3.avi265KSupporting info item
AJT_4067_sm_MOVIE_4.avi375KSupporting info item
AJT_4067_sm_MOVIE_5.avi254KSupporting info item
AJT_4067_sm_MOVIE_6.avi242KSupporting info item

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