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Supplemental Figure I: The potential of multilineage differentiation of MSCs cultured from rat bone marrow. (A) Adipogenic differentiation of rat MSCs was evidenced by the formation of lipid vacuoles in phase-contrast photograph (left) and by oil-red O staining (right). (B) Osteogenic differentiation of rat MSCs was evidenced by mineral deposition demonstrated by Alizarin Red staining (right). Scale bars: 50 μm.

Supplemental Figure II: The expression of target genes in infected cells. (A) Recombinant lentiviral vectors were generated carrying specific SM22α promoter and the downstream EGFP with or without p53 gene. Lentiviral particles were prepared and used to infect VSMCs and endothelial cells. Fluorescence microscopy of the infected cells was performed 48 h after infection. Transfection efficiency was greater than 90%. SM22α promoter modulated GFP expression in VSMCs, but not in endothelial cells. Original magnification: ×100. (B) mRNA expression of p53 was determined by quantitative RT-PCR in infected and uninfected VSMCs. mRNA levels are normalized to GAPDH. (C) Western blot analysis showed increased p53 protein levels in Ltv-p53 infected VSMCs compared with Ltv-vector-infected VSMCs. Representative western blot (upper panel) of three independent experiments. Densitometric analyses (lower panel) are presented as the relative ratio of each protein to GAPDH. Data are shown as mean ± SEM (n = 3). ** p < 0.01 versus Ltv-vector.

Supplemental Figure III: The expression of target genes in aortic grafts. (A) Aortic grafts were harvested from isograft and allograft rats one week after surgery. Frozen sections were prepared and assessed by fluorescence microscopy. GFP expression confined to aortic media is noted in sections from Ltv-vector and Ltv-BclxL allografts. Sections from an uninfected isograft only show elastic fiber auto-fluorescence. Scale bar: 200 μm. (B) mRNA expression of BclxL was determined by quantitative RT-PCR in aortic grafts from isograft and allograft rats two weeks after surgery. mRNA levels are normalized to GAPDH. Data are shown as mean ± SEM (n = 3). ** p < 0.01 versus Ltv-vector.

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AJT_4082_sm_SuppMat.doc76KSupporting info item
AJT_4082_sm_FigureI.doc318KSupporting info item
AJT_4082_sm_FigureII.doc232KSupporting info item
AJT_4082_sm_FigureIII.doc82KSupporting info item

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