These authors contributed equally to this work.
Leptin Modulates Allograft Survival by Favoring a Th2 and a Regulatory Immune Profile
Article first published online: 27 SEP 2012
© Copyright 2012 The American Society of Transplantation and the American Society of Transplant Surgeons
American Journal of Transplantation
Volume 13, Issue 1, pages 36–44, January 2013
How to Cite
Moraes-Vieira, P. M. M., Bassi, E. J., Larocca, R. A., Castoldi, A., Burghos, M., Lepique, A. P., Quintana, F. J., Araujo, R. C., Basso, A. S., Strom, T. B. and Câmara, N. O. S. (2013), Leptin Modulates Allograft Survival by Favoring a Th2 and a Regulatory Immune Profile. American Journal of Transplantation, 13: 36–44. doi: 10.1111/j.1600-6143.2012.04283.x
- Issue published online: 26 DEC 2012
- Article first published online: 27 SEP 2012
- Manuscript Accepted: 17 AUG 2012
- Manuscript Revised: 17 AUG 2012
- Manuscript Received: 10 APR 2012
- State of Sao Paulo Foundation for Research Support. Grant Numbers: 07/07139-3, 08/55447-1, 09/50450-7, 08/58564-9, 10/52180-4, 12/02270-2
- International Associated Laboratory in Renal Immune RegulationImmuneregulation
- Brazilian Council of Scientific and Technologic Development. Grant Number: 470533/2007-2
- Complex Fluids INCT
Vol. 13, Issue 8, 2231, Article first published online: 26 JUL 2013
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|ajt4283-sup-0001-suppmatS1.pdf||175K||Figure S1: Frequency of lymph node CD4+ and CD8+ T cells in the lymph nodes of nonmanipulated B6 Lepob/ob and control mice. The cells from the draining lymph nodes were obtained, stained with anti-CD4 (A) and anti-CD8 (B) monoclonal antibodies and analyzed by flow cytometry (n = 5 mice/group). Data are representative of two independent experiments. The results are shown as the mean ± SEM. No statistical differences were observed among the groups.|
|ajt4283-sup-0002-suppmatS2.pdf||124K||Figure S2: IL-17 does not influence fully mismatched skin allograft survival. CBA skin was transplanted onto normal or IL-17 KO B6 mice. Signs of allograft rejection were monitored daily, and survival is reported for individual animals as the time of graft rejection (n = 4 animals/group). The data are representative of two independent experiments.|
|ajt4283-sup-0003-suppmatS3.pdf||42K||Figure S3: Tregs and Th17 cells from Lepob/ob naïve T cells are more effectively induced than those from WT naïve T cells. Naïve (CD4+CD62L+CD44−CD25−) T cells from wild-type or Lepob/ob B6 mice were cultured for 5 days in the presence or absence of TGF-β, IL-6 and leptin. Treg and Th17 differentiation of naïve CD4+ T cells from WT and Lepob/ob mice. The induction of Foxp3+ CD4+ T cells and IL-17+ CD4+ T cells was evaluated through the intracellular staining of Foxp3 and IL-17, respectively. The data are representative of three independent experiments. Lepob/ob: CD4+ T cells from Lepob/ob cultured in the absence of leptin; Lepob/obLep: CD4+ T cells from Lepob/ob cultured in the presence of recombinant leptin; WT: CD4+ T cells from wild-type mice. Data are represented from three independent experiments. The results are shown as the mean ± SEM. **p < 0.01.|
|ajt4283-sup-0004-suppmatS4.pdf||140K||Figure S4: CD4+ T cell frequency in RAG−/- skin-transplanted mice. A total of 5×.106 purified CD4+ T cells from wild-type, Lepob/ob or Lepdb/db mice were transferred to skin-grafted RAG−/− mice. At the time of the last graft rejection, the lymph nodes were obtained and stained with an anti-CD4 antibody. The cells were analyzed by flow cytometry. The data are representative of two independent experiments. (n = 3 mice/group).|
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