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Keywords:

  • dental materials;
  • root filling materials;
  • human fibroblasts;
  • cell culture;
  • toxicity

Abstract Standardized test tubes filled with freshly mixed root filling materials (AH26, CRCS, N2, Kloroperka NØ, ZOE cement and 2 experimental cements, ECI and ECII) were transferred into tissue culture flasks. Normal human oral fibroblasts were seeded in the flasks. Morphological cell changes were studied up to 15 days after seeding. The size of cell-free zones around the test tubes and the total cell number per culture flask were calculated after 5, 10 and 15 days. The findings showed N2 cement to be by far the most toxic material at all observation periods, whereas no toxic reactions could be seen in relation to tubes filled with Kloroperka NØ. Compared with the 5-clay observation period, some cell recovery was observed around test tubes with AH26 and ECII, whereas almost full cell recovery was found around test tubes with CRCS, ZOE and ECI. It was concluded that the present model, which allows long-term observations of human cellular reactions to dental materials, can be used as a simple and relatively cheap screening test for initial toxicity testing of dental materials.