Mechanical removal of necrotic periodontal ligament by either Robinson bristle brush with pumice or scalpel blade. Histomorphometric analysis and scanning electron microscopy

Authors

  • Helen Ramon Esper,

    1. Discipline of Integrated Clinics, Department of Surgery and Integrated Clinics, Faculty of Dentistry of Araçatuba, UNESP, Araçatuba
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  • Sônia Regina Panzarini,

    1. Discipline of Integrated Clinics, Department of Surgery and Integrated Clinics, Faculty of Dentistry of Araçatuba, UNESP, Araçatuba
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  • Wilson Roberto Poi,

    1. Discipline of Integrated Clinics, Department of Surgery and Integrated Clinics, Faculty of Dentistry of Araçatuba, UNESP, Araçatuba
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  • Celso Koogi Sonoda,

    1. Discipline of Integrated Clinics, Department of Surgery and Integrated Clinics, Faculty of Dentistry of Araçatuba, UNESP, Araçatuba
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  • Cláudio Aparecido Casatti

    1. Discipline of Histology, Department of Basic Sciences, Faculty of Dentistry of Araçatuba, UNESP, Araçatuba, SP, Brazil
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Dra. Helen Ramon Esper, Departamento de Cirurgia e Clínica, Integrada, Faculdade de Odontologia do Campus de Araçatuba, UNESP, Rua José Bonifácio, 1193, 1601-050 Araçatuba, SP, Brasil
Tel.: +55 18 3636 3240
Fax: +55 18 3636 3332
e-mail: helen_esper@hotmail.com

Abstract

Abstract –  One of the important factors accounting for successful delayed replantation of avulsed teeth is seemingly the type of root surface treatment. Removal of necrotic cemental periodontal ligament remnants may prevent the occurrence of external root resorption, which is the major cause of loss of teeth replanted in such conditions. The purpose of this study was to compare the efficacy of two mechanical techniques for removal of root-adhered periodontal ligament. Preservation or removal of the cementum layer concomitantly with these procedures was also assessed. Forty-five roots of healthy premolars extracted for orthodontic purposes were selected. After extraction, the teeth were kept dry at room temperature for 1 h and then immersed in saline for rehydration for an additional 10 min. Thereafter, the roots were assigned to three groups, as follows: group 1 (control) - the cemental periodontal ligament was preserved; group 2 - removal of the periodontal ligament by scraping root surface with a scalpel blade (SBS); group 3 - periodontal ligament remnants were removed using a Robinson bristle brush at low-speed with pumice/water slurry (RBP). The specimens were analysed histomorphometrically and examined by scanning electron microscopy. The quantitative and qualitative analyses of the results showed that the RBP technique was significantly more effective than the SBS technique for removal of the periodontal ligament remnants adhered to root surface. Both techniques preserved the cementum layer.

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