Abstract – The type of storage medium used to store avulsed teeth prior to replantation has been shown to be a decisive factor in periodontal ligament (PDL) healing. The aim of the present study was to investigate the effect of storage medium on periodontal healing. Thirty teeth from three dogs were endodontically treated to prevent subsequent inflammatory root resorption. The teeth were atraumatically extracted and randomly stored in milk or egg albumen for 3, 6 and 10 h at 4°C. All teeth were splinted for 1 weeks after replanting. After 2 months animals were sacrificed using vital perfusion-fixation and teeth were histologically prepared and evaluated following Andreasen’s method. It was found that teeth stored in egg albumen for 6 and 10 h had significantly higher incident of PDL healing than those treated with milk for the same period (P < 0.05). . The highest incidence of PDL healing was observed in teeth stored in egg albumen for 6 h. The least surface resorption was also evident in this group (P < 0.05). The result of this study shows that egg albumen is an excellent storage media for up to 10 h considering its likely availability at most accident sites.