The first two authors contributed equally to this work.
Alteration of BMP-4 and Runx2 expression patterns in mouse temporomandibular joint after ovariectomy
Article first published online: 31 AUG 2006
Volume 13, Issue 2, pages 220–227, March 2007
How to Cite
Min, H.-J., Lee, M.-J., Kim, J.-Y., Cho, S.-W., Park, H.-D., Lee, S.-I., Kim, H.-J. and Jung, H.-S. (2007), Alteration of BMP-4 and Runx2 expression patterns in mouse temporomandibular joint after ovariectomy. Oral Diseases, 13: 220–227. doi: 10.1111/j.1601-0825.2006.01270.x
- Issue published online: 25 JAN 2007
- Article first published online: 31 AUG 2006
- Received 23 December 2005; revised 24 February 2006; accepted 6 March 2006
- temporomandibular joint;
- temporomandibular disorder;
Objective: Temporomandibular disorder (TMD) includes a number of clinical conditions involving the masticatory musculature or the temporomandibular joint (TMJ) and associated structures. Previous studies have shown the presence of high-affinity estrogen receptors in the TMJ articular cartilage. The aim of this study was to evaluate the developmental changes in mouse TMJ under estrogen deficiency.
Materials and methods: Four-month-old ovariectomized mice were killed after certain weeks. We examined the significant alterations of the expression patterns of bone morphogenetic protein (BMP)-4, Runx2, and bone sialoprotein (BSP) after ovariectomy.
Results: In the control group, BMP-4, Runx2, and BSP expressions showed no definite difference at any stage. In the ovariectomy group, the intensity of BMP-4 and Runx2 expression increased after ovariectomy. BSP immunoreactivity, however, increased slightly at 2 weeks but then decreased gradually.
Conclusions: Estrogen plays important roles in the metabolism and maintenance of TMJ via regulations of signaling molecules such as BMP-4, Runx2, and BSP. Our results suggest that estrogen deficiency is a candidate cause of TMD. This study revealed further osteogenetic properties of estrogen that may be useful in the clinical treatment and prevention of TMD.