Non-surgical periodontal treatment of cyclosporin A-induced gingival overgrowth: immunohistochemical results

  1. M Aimetti1,
  2. F Romano1,
  3. A Marsico2,
  4. R Navone2

Article first published online: 10 FEB 2008

DOI: 10.1111/j.1601-0825.2007.01364.x

Issue

Oral Diseases

Oral Diseases

Volume 14, Issue 3, pages 244–250, April 2008

Additional Information

How to Cite

Aimetti, M., Romano, F., Marsico, A. and Navone, R. (2008), Non-surgical periodontal treatment of cyclosporin A-induced gingival overgrowth: immunohistochemical results. Oral Diseases, 14: 244–250. doi: 10.1111/j.1601-0825.2007.01364.x

Author Information

  1. 1

    Sections of Periodontology

  2. 2

    Pathology, Department of Medical Sciences and Human Oncology, University of Torino, Torino, Italy

*Dr M Aimetti, Corso Marconi, 13 10125 Torino, Italy. Tel: +390116503147, Fax: +390116682286, E-mail: mario.aimetti@unito.it

Publication History

  1. Issue published online: 10 FEB 2008
  2. Article first published online: 10 FEB 2008
  3. Received 20 September 2006; revised 27 November 2006; accepted 10 December 2006

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Keywords:

  • cyclosporin-A gingival overgrowth;
  • gingival histology;
  • immunohistochemistry;
  • non-surgical periodontal treatment;
  • scaling

Aim:  The present study was planned to analyze the effects of a 12-month non-surgical periodontal treatment on histologic and immunohistochemical features of cyclosporin A (CsA)-induced gingival overgrowth (GO).

Materials and methods:  Gingival samples were collected from 21 liver transplant subjects exhibiting CsA-induced GO prior to, and 12 months after non-surgical periodontal therapy including oral hygiene instructions, scaling and 2-month recall appointments, and also from 18 healthy control subjects. Gingival biopsy specimens were stained with hematoxylin–eosin and monoclonal antibodies for vimentin, CD3 (T-lymphocytes), CD20 (B-lymphocytes), CD34 (endothelium) and Ki-67 (fibroblasts proliferation rate), using a streptavidin-biotin-peroxidase complex method.

Results:  Total inflammatory cells, gingival vessels and fibroblast proliferation rate demonstrated significant reduction after non-surgical periodontal treatment (P < 0.0001) in overgrown gingiva, while B- and T-lymphocytes remained nearly unchanged (P = 0.61 and 0.33, respectively). At the 12-month evaluation no significant differences were found when comparing the gingival biopsies from CsA-treated patients and those from healthy controls (P > 0.05).

Conclusions:  Control of clinical inflammation by means of non-surgical periodontal treatment results both in lowering of inflammatory infiltrate and in changes in connective tissue composition. Thus, plaque-induced inflammation would seem to modulate the drug-gingival tissue interaction.

Clinical relevance:  A strict plaque control program play a pivotal role in the management of transplant patients exhibiting cyclosporin A-GO.

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