SHED repair critical-size calvarial defects in mice

  1. BM Seo1,
  2. W Sonoyama2,
  3. T Yamaza2,
  4. C Coppe3,
  5. T Kikuiri2,
  6. K Akiyama2,
  7. JS Lee3,
  8. S Shi2

Article first published online: 28 JUN 2008

DOI: 10.1111/j.1601-0825.2007.01396.x

Issue

Oral Diseases

Oral Diseases

Volume 14, Issue 5, pages 428–434, July 2008

Additional Information

How to Cite

Seo, B., Sonoyama, W., Yamaza, T., Coppe, C., Kikuiri, T., Akiyama, K., Lee, J. and Shi, S. (2008), SHED repair critical-size calvarial defects in mice. Oral Diseases, 14: 428–434. doi: 10.1111/j.1601-0825.2007.01396.x

Author Information

  1. 1

    Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Seoul, Korea

  2. 2

    Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, CA, USA

  3. 3

    Department of Oral & Maxillofacial Surgery, University of California at San Francisco, San Francisco, CA, USA

*Dr S Shi, Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA. Tel: +1 323 442 3038, Fax: +1 323 442 2981, E-mail: songtaos@usc.edu

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008
  3. Received 18 January 2007; revised 8 March 2007; accepted 14 March 2007
Corrected by:

Erratum

Vol. 15, Issue 4, 302, Article first published online: 8 APR 2009

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Keywords:

  • stem cells from human exfoliated deciduous teeth (SHED);
  • osteoblast;
  • regeneration;
  • bone

Objective:  Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice.

Materials and methods:  We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas.

Results:  SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential.

Conclusions:  Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.

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