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Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts
Article first published online: 29 JUN 2009
DOI: 10.1111/j.1601-0825.2009.01590.x
© 2009 John Wiley & Sons A/S
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How to Cite
Gadbail, A., Chaudhary, M., Patil, S. and Gawande, M. (2009), Actual Proliferating Index and p53 protein expression as prognostic marker in odontogenic cysts. Oral Diseases, 15: 490–498. doi: 10.1111/j.1601-0825.2009.01590.x
Publication History
- Issue published online: 10 SEP 2009
- Article first published online: 29 JUN 2009
- Received 4 March 2009; revised 11 April 2009; accepted 27 April 2009
- Abstract
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Keywords:
- Actual Proliferating Index;
- odontogenic keratocysts/keratocystic odontogenic tumour;
- radicular cysts;
- dentigerous cyst;
- Ki-67 Labelling Index;
- p53 Labelling Index;
- argyrophilic nucleolar organizer regions
Background: The purpose of this study was to evaluate the biological aggressiveness of odontogenic keratocyst/keratocystic odontogenic tumour (KCOT), radicular cyst (RC) and dentigerous cyst (DC) by observing the actual proliferative activity of epithelium, and p53 protein expression.
Methods: The actual proliferative activity was measured by Ki-67 Labelling Index and argyrophilic nucleolar organizing regions (AgNOR) count per nucleus. The p53 protein expression was also evaluated.
Results: Ki-67 positive cells were observed higher in suprabasal cell layers of KCOT with uniform distribution, a few of them were predominantly observed in basal cell layer in RC and DC. The AgNOR count was significantly higher in suprabasal cell layers of KCOT. The actual proliferative activity was noted to be higher in suprabasal cell layers of KCOT. The p53 immunolabelling was dense and scattered in basal and suprabasal cell layers in KCOT. The weakly stained p53 positive cells were observed diffusely distributed in KCOT, whereas they were mainly seen in basal cell layer of RC and DC.
Conclusion: The quantitative and qualitative differences of the proliferative activity and the p53 protein expression in sporadic KCOT may be associated with intrinsic growth potential that could play a role in its development and explain locally aggressive biological behaviour. AgNOR count and p53 protein detection in odontogenic lesions can be of great consequence to predict the biological behaviour and prognosis.