LINE-1 methylation difference between ameloblastoma and keratocystic odontogenic tumor
Article first published online: 15 MAR 2010
© 2010 John Wiley & Sons A/S
Volume 16, Issue 3, pages 286–291, April 2010
How to Cite
Kitkumthorn, N. and Mutirangura, A. (2010), LINE-1 methylation difference between ameloblastoma and keratocystic odontogenic tumor. Oral Diseases, 16: 286–291. doi: 10.1111/j.1601-0825.2009.01640.x
- Issue published online: 15 MAR 2010
- Article first published online: 15 MAR 2010
- Received 19 August 2009; revised 21 September 2009; accepted 30 September 2009
- keratocystic odontogenic tumor;
- methylation level;
Oral Diseases (2010) 16, 286–291
Objective: Global hypomethylation is a common epigenetic event in cancer. Keratocystic odontogenic tumor (KCOT) and ameloblastoma are different tumors but posses the same tissue in origin. Here, we investigated long interspersed nuclear element-1 (LINE-1 or L1) methylation status between ameloblastoma and KCOT.
Materials and methods: We studied the methylation levels of the long interspersed nucleotide element-1 (LINE-1) in ameloblastoma and KCOT. After collecting ameloblastoma cells and epithelium lining cells of KCOT by laser capture microdissection from paraffin embedded tissue, combined bisulfite restriction analysis of LINE-1 (COBRALINE-1) was performed to measure LINE-1 methylation levels.
Results: The LINE-1 methylation level in KCOT (53.16 ± 12.03%) was higher than that in ameloblastoma (36.90 ± 16.52%), with a statistical significance of P = 0.001. The ranges of LINE-1 methylation of both lesions were not associated with either age or sex.
Conclusion: We found LINE-1 hypomethylation levels between ameloblastoma and KCOT are different. Therefore, global methylations between these tumors are processed differently.