Altered expression of mRNA for HIF-1α and its target genes RTP801and VEGF in patients with oral lichen planus

  1. M Ding1,2,†,
  2. JY Xu3,
  3. Y Fan3,†

Article first published online: 15 MAR 2010

DOI: 10.1111/j.1601-0825.2009.01645.x

Issue

Oral Diseases

Oral Diseases

Volume 16, Issue 3, pages 299–304, April 2010

Additional Information

How to Cite

Ding, M., Xu, J. and Fan, Y. (2010), Altered expression of mRNA for HIF-1α and its target genes RTP801and VEGF in patients with oral lichen planus. Oral Diseases, 16: 299–304. doi: 10.1111/j.1601-0825.2009.01645.x

Author Information

  1. 1

    Department of Stomatology, Suzhou Health College, Suzhou, Jiangsu, China

  2. 2

    Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL, USA

  3. 3

    The Research Institute of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, China

  1. †

    These authors contributed equally to this work.

*Yuan Fan, DDS, PhD, The Research Institute of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing 210029, China. Tel: +86 025 85031817, Fax: +86 025 86516414, E-mail: fanyuan65@hotmail.com

  • †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 15 MAR 2010
  2. Article first published online: 15 MAR 2010
  3. Received 27 March 2009; revised 25 October 2009; accepted 18 October 2009

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Keywords:

  • HIF-1α;
  • RTP801;
  • VEGF;
  • oral lichen planus

Oral Diseases (2010) 16, 299–304

Objective:  To explore a potential causal contribution of the transcription factor HIF-1α and its target gene, RTP801 and VEGF, to the development of oral lichen planus (OLP).

Design relevant:  Twenty-two adult OLP patients were enrolled in this study. All OLP diagnoses were verified by histopathological characteristics. Normal mucous specimens were collected from 12 controls after various oral surgeries.

Material and method:  RNA was isolated from OLP and control specimens. Microarray was performed using BiostarH-40s gene chip. Expression of HIF-1α, VEGF and RTP801 was evaluated using quantitative real-time polymerase chain reaction (qPCR). Unpaired t-test and one-way ANOVA was used for statistical analysis.

Results:  Microarray results showed that RTP801 expression was lower in OLP than in controls (779 vs 3090). qPCR further confirmed that expression of RTP801 was similarly lower in OLP than in controls (0.363 vs 1.473, P < 0.001); expression of VEGF was also lower in OLP (0.448 vs 1.74, P = 0.012). In contrast, expression of HIF-1α was higher in OLP than in controls (11.12 vs 1.628, P < 0.001).

Conclusion:  The oral mucosa of OLP is hypoxic. Genes that are activated by hypoxia, such as RTP801 and VEGF, and their signal cascades may be novel potential therapeutic targets for OLP.

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