A preliminary study on the FAM5C expression in generalized chronic periodontitis
Article first published online: 3 OCT 2011
© 2011 John Wiley & Sons A/S
Volume 18, Issue 2, pages 147–152, March 2012
How to Cite
Ribeiro, F., Santos, V., Bastos, M., de Miranda, T., Vieira, A., de Figueiredo, L. and Duarte, P. (2012), A preliminary study on the FAM5C expression in generalized chronic periodontitis. Oral Diseases, 18: 147–152. doi: 10.1111/j.1601-0825.2011.01855.x
- Issue published online: 16 JAN 2012
- Article first published online: 3 OCT 2011
- Accepted manuscript online: 8 SEP 2011 06:36AM EST
- Received 6 June 2011; revised 16 August 2011; accepted 24 August 2011
- chronic periodontitis;
- FAM5C protein, human;
- polymerase chain reaction
Oral Diseases (2012) 18, 147–152
Objective: The Family with sequence similarity 5 member C (FAM5C) has been suggested to contribute in aggressive periodontitis. However, there is no data regarding its role in chronic periodontitis. The aim of this study was to evaluate the FAM5C expression in chronic periodontitis and to study association of FAM5C with key immunoinflammatory markers.
Material and Methods: Gingival biopsies were harvested from periodontally healthy subjects (n = 10) and chronic periodontitis subjects (n = 15). The levels of mRNA of FAM5C, interleukin (IL)-17, IL-6, IL-23, IL-10, IL-4, interferon-γ, toll-like receptor (TLR)-2, TLR-4, osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), tumor necrosis factor (TNF)-α, transforming growth factor-β, transcription factor forkhead box p3, and transcription factor orphan nuclear receptor C2 were evaluated by real-time polymerase chain reaction.
Results: FAM5C mRNA levels were not different between periodontally healthy and diseased tissues (P > 0.05). Gene expressions of IL-17, TNF-α, OPG, RANKL, TLR-2, and TLR-4 were higher in periodontitis, when compared to periodontally healthy sites (P < 0.05), while no differences between groups were observed for the other genes evaluated (P > 0.05). There were no correlations between the gene expression of FAM5C and the other immunoinflammatory markers (P > 0.05).
Conclusion: Within the limits of this study, it seems that FAM5C expression does not contribute to chronic periodontitis.