Enhanced alveolar bone loss in a model of non-invasive periodontitis in rice rats

Authors


J. Ignacio Aguirre, DVM, PhD, Department of Physiological Sciences, Box 100144, JHMHC, University of Florida, Gainesville, FL 32610, USA. Tel: (352) 273-6079, Fax: (352) 392-5145, E-mail: aguirrej@ufl.edu

Abstract

Oral Diseases (2012) 18, 459–468

Objective:  The rice rat (Oryzomys palustris) develops periodontitis-like lesions when fed a diet rich in sucrose and casein (H-SC). We aimed to establish whether this model can accurately mimic the development of human periodontitis.

Materials and Methods:  For this purpose, 28-day-old rice rats (15/group) were assigned to standard (STD) or H-SC diets and sacrificed after 6, 12, and 18 weeks. Jaws were processed for morphometric, histometric, histologic, histomorphometric, and micro-CT analyses.

Results:  We found a progressive increase in horizontal alveolar bone loss (ABL) with age in maxillae of rats fed the STD diet as determined by morphometry. The H-SC diet exacerbated horizontal ABL at the palatal surface at 12 and 18 weeks. Furthermore, increased vertical ABL was detected in mandibles and maxillae of rats fed the H-SC diet for 12 and/or 18 weeks by histometry and micro-CT. Remarkably, the H-SC diet significantly increased bone remodeling at the interproximal alveolar bone of mandibles from rats fed for 6 weeks, but not in those fed for longer periods.

Conclusions:  These findings indicate that the H-SC diet induced a transient increase in alveolar bone remodeling, which is followed by ABL characteristic of moderate periodontitis.

Ancillary