Chitinase expression in parotid glands of non-obese diabetic mice
Article first published online: 6 FEB 2012
© 2012 John Wiley & Sons A/S
Volume 18, Issue 5, pages 506–512, July 2012
How to Cite
Fukushima, T., Nashida, T., Haga-Tsujimura, M. and Mataga, I. (2012), Chitinase expression in parotid glands of non-obese diabetic mice. Oral Diseases, 18: 506–512. doi: 10.1111/j.1601-0825.2012.01904.x
- Issue published online: 6 JUN 2012
- Article first published online: 6 FEB 2012
- Accepted manuscript online: 10 JAN 2012 12:16PM EST
- Received 12 October 2011; revised 13 December 2011; accepted 1 January 2012
- NOD mouse
Oral Diseases (2012) 18, 506–512
Objective: This investigation was a basal study that used a mouse model of xerostomia to identify protein biomarkers of xerostomia in saliva. We identified genes expressed differently in parotid glands from non-obese diabetic mice with diabetes and those from control mice; subsequently, we investigated expression of the proteins encoded by these genes in parotid glands and saliva.
Materials and Methods: DNA microarray and real-time PCR analyses were performed to detect differences between NOD/ShiJcl and C57BL/6JJcl (control) female mice in gene expression from parotid glands or parotid acinar cells. Subsequently, protein expression was assessed using immunoblotting and immunohistochemistry. Similarly, enzyme activity in saliva was assessed using zymography.
Results: Based on gene expression analyses, Chia expression was higher in diabetic mice than non-diabetic mice and control mice; similarly, expression of chitinase, the protein encoded by Chia, was higher in diabetic mice. Saliva from NOD/ShiJcl mice had more chitinase than saliva from control mice.
Conclusions: Chitinase was highly expressed in parotid acinar cells from diabetic mice compared with non-diabetic and control mice. Increased chitinase expression and enzyme activity may characterize the autoimmune diabetes in mice; however, further investigation is required to assess its use as a biomarker of xerostomia in humans.