Stimulation of bone formation in the expanding premaxillary suture with a GSK-3β inhibitor
Article first published online: 8 JUL 2012
© 2012 John Wiley & Sons A/S
Volume 19, Issue 1, pages 73–79, January 2013
How to Cite
Jiang, Y., Liu, H., Guo, J., Tang, G. and Qian, Y. (2013), Stimulation of bone formation in the expanding premaxillary suture with a GSK-3β inhibitor. Oral Diseases, 19: 73–79. doi: 10.1111/j.1601-0825.2012.01957.x
- Issue published online: 7 DEC 2012
- Article first published online: 8 JUL 2012
- Accepted manuscript online: 15 JUN 2012 11:45AM EST
- Received 10 April 2012; revised 27 May 2012; accepted 3 June 2012
- premaxillary suture;
Oral Diseases (2012) 19, 73–79
Objectives: Glycogen synthase kinase-3β (GSK-3β)/β-catenin signaling mediates osteogenesis in response to mechanical loading. We tested the hypothesis that local administration of a GSK-3β inhibitor could stimulate new bone formation in the expanding premaxillary suture.
Materials and methods: Thirty-five Sprague–Dawley rats were subjected to premaxillary suture expansion using a helix spring. The experimental rats were given one or two local injections of SB-415286, a small-molecule GSK-3β inhibitor. Animals were administered calcein and sacrificed on day 7 to quantify new bone formation. To evaluate the proliferation and differentiation of osteoblasts, rats were labeled with bromodeoxyuridine on day 1 and sacrificed on day 2 or 4. β-catenin expression was evaluated by immunohistochemical staining.
Results: Two injections of SB-415286 led to an elevation of β-catenin expression and an increase in the number of proliferating osteoblasts in expanding sutures on day 2 and day 4. Consequently, new bone formation in the suture increased significantly on day 7.
Conclusions: These results suggest that local delivery of a GSK-3β inhibitor could stimulate bone formation in the expanding premaxillary suture by eliciting β-catenin signaling. GSK-3β could be a pharmaceutical target for improving the effect of orthodontic treatments such as rapid palatal expansion.