Immunophenotypic characterization and distribution of dendritic cells in odontogenic cystic lesions
Article first published online: 13 JUL 2012
© 2012 John Wiley & Sons A/S
Volume 19, Issue 1, pages 85–91, January 2013
How to Cite
Matos, F., Rizo, V., Almeida, L., Tirapelli, C., Silva-Sousa, Y., Almeida, O. and León, J. (2013), Immunophenotypic characterization and distribution of dendritic cells in odontogenic cystic lesions. Oral Diseases, 19: 85–91. doi: 10.1111/j.1601-0825.2012.01960.x
- Issue published online: 7 DEC 2012
- Article first published online: 13 JUL 2012
- Accepted manuscript online: 28 JUN 2012 07:50AM EST
- Received 10 April 2012; revised 26 May 2012; accepted 12 June 2012
- odontogenic cysts;
- jaws, oral;
- dendritic cells;
Oral Diseases (2012) 19, 85–91
Objective: To analyze the expression and distribution patterns of mature dendritic cells (mDCs) and immature DCs (imDCs) in radicular cysts (RCs), dentigerous cysts (DtCs), and keratocystic odontogenic tumors (KCOTs).
Materials and methods: Forty-nine odontogenic cystic lesions (OCLs) (RCs, n = 20; DtCs, n = 15; KCOTs, n = 14) were assessed using the following markers: S100, CD1a and CD207 for imDCs; and CD83 for mDCs.
Results: Almost all cases were S100, CD1a, and CD207 positive, whereas 63% were CD83 positive. RCs presented greater number of immunostained cells, followed by DtCs, and KCOTs. The number of S100+ cells was greater than both CD1a+ and CD207+ cells (P < 0.001), which showed approximately similar amounts, followed by lower number of CD83+ cells (P < 0.001) in each OCL type. Different from S100+ cells, both CD1a+ and CD207+ cells on the epithelium (P < 0.05) and CD83+ cells on the capsule (P < 0.05) were preferentially observed. In RCs, significant correlation was found between the thickness epithelium with S100+ and CD1a+ cells, and between the degree of inflammation with CD83+ cells.
Conclusions: Dendritic cell populations in OCLs can be phenotypically heterogeneous, and it could represent distinct lineages and/or functional stages. It is suggested that besides DC-mediated immune cell interactions, DC-mediated tissue differentiation and maintenance in OCLs should also be considered.