Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts

Authors

  • A Zizzi,

    1. Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of the Marche/United Hospitals, Torrette (Ancona)
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    • Equally contributed to this work.

  • SD Aspriello,

    1. Department of Clinical Specialistic and Dental Sciences, Periodontology, Polytechnic University of the Marche, Torrette (Ancona)
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    • Equally contributed to this work.

  • L Ferrante,

    1. Department of Biomedical Sciences and Public Health, Centre of Epidemiology, Biostatistic and Medical Informatics, Polytechnic University of the Marche, Torrette (Ancona)
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  • D Stramazzotti,

    1. Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of the Marche/United Hospitals, Torrette (Ancona)
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  • G Colella,

    1. Unit of Maxillofacial Surgery Head and Neck Department, II University of Napoli, Napoli
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  • P Balercia,

    1. Department of Surgical and Medical Neurologic Sciences, Division of Maxillofacial Surgery, Polytechnic University of the Marche, Torrette (Ancona)
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  • L Lo Muzio,

    1. Department of Surgical Sciences, University of Foggia, Foggia, Italy
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  • M Piemontese,

    1. Department of Clinical Specialistic and Dental Sciences, Periodontology, Polytechnic University of the Marche, Torrette (Ancona)
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  • G Goteri,

    1. Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of the Marche/United Hospitals, Torrette (Ancona)
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  • C Rubini

    1. Department of Biomedical Sciences and Public Health, Section of Pathologic Anatomy and Histopathology, Polytechnic University of the Marche/United Hospitals, Torrette (Ancona)
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Gaia Goteri, Section of Pathologic Anatomy and Histopathology, Department of Biomedical Sciences and Public Health, Polytechnic University of the Marche/United Hospitals, Via Conca, 71, 60020 Torrette (Ancona), Italy. Tel. +390715964811, Fax: +39071889985, E-mail: g.goteri@univpm.it

Abstract

Oral Diseases (2012) 19, 92–99

Objective:  Radicular cysts occur as a result of the immunological response to continuous antigenic stimulation from root canals. We correlated the immunophenotypical composition of the lymphoid infiltrate to the microvessel density expressed by the count of CD34 reactive endothelial cells in radicular cysts.

Subjects and methods:  Thirty-four cases of radicular cysts were evaluated by immunohistochemistry, using antibodies against B- and T-cell antigens (CD20, CD3, CD4, CD8) and against the endothelial cell marker CD34. Statistical analysis was performed.

Results:  In the epithelium, we observed a low amount of lymphoid infiltrate in all 34 radicular cysts, and a strong significant negative correlation between T and B lymphocytes and between T-helper and T-cytotoxic/suppressor lymphocytes. In the cyst capsule, we observed a significant positive correlation between B and T lymphocytes, B and T-cytotoxic/suppressor lymphocytes, T and T-helper lymphocytes and between the number of CD34+ blood vessels and T and T-helper lymphocytes, respectively. We observed a statistically significant correlation between percentage of CD34+ vessels and inflammatory infiltrate grade.

Conclusions:  Both humoral and cellular immune reactions and neovascularization are likely to occur in the complex events of tissue destruction. The inflammatory infiltrate has an important role in neoangiogenesis and consequently in radicular cysts development and growth.

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