Amygdala protein kinase C epsilon controls alcohol consumption

Authors

  • H. M. B. Lesscher,

    Corresponding author
    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
      H. M. B. Lesscher, Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands. E-mail: H.M.B.Lesscher@umcutrecht.nl
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      Present address: Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands
  • M. J. Wallace,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • L. Zeng,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • V. Wang,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • J. K. Deitchman,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • T. McMahon,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • R. O. Messing,

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • P. M. Newton

    1. Department of Neurology, Ernest Gallo Clinic and Research Center, University of California at San Francisco, Emeryville, CA, USA
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  • Note: Outstanding Young Investigators Awardee, 9th Annual Meeting of the International Behavioural and Neural Genetics Society,Doorwerth, The Netherlands, May 21–25, 2007.

    Present address: Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands

H. M. B. Lesscher, Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands. E-mail: H.M.B.Lesscher@umcutrecht.nl

Abstract

Alcoholism is a progressive disorder that involves the amygdala. Mice lacking protein kinase C epsilon (PKCɛ) show reduced ethanol consumption, sensitivity and reward. We therefore investigated whether PKCɛ signaling in the amygdala is involved in ethanol consumption. Local knockdown of PKCɛ in the amygdala reduced ethanol consumption and preference in a limited-access paradigm. Further, mice that are heterozygous for the PKCɛ allele consume less ethanol compared with wild-type mice in this paradigm. These mice have a >50% reduction in the abundance of PKCɛ in the amygdala compared with wild-type mice. We conclude that amygdala PKCɛ is important for ethanol consumption in mice.

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