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Supporting Information

Additional Supporting Information may be found in the online version of this article:

Movie S1: Short latency startle responses to acoustic stimuli in sibling (a) and twitch twice/robo3 mutant larvae (b). Movies are 100-ms duration and slowed 20 times. Time is represented by the blue dot at the bottom of the video, with the position of the stimulus indicated. Orange crosses indicate the position of the eyes as automatically determined by the analysis software. Although wild-type larvae remain upright throughout the startle response, mutant larvae frequently roll and become horizontally oriented or inverted.

Movie S2: O-bend responses to Dark Flash stimuli in sibling (a) and twitch twice/robo3 mutant larvae (b). Movies are 400-ms duration and slowed 10 times. Larval curvature at each time-point is represented by the red dot at the bottom of the video. Wild-type larvae perform a stereotyped fast large angle bend, followed by a counterbend of similar magnitude. In contrast, both the initial bend and counterbend in twitch twice/robo3 mutants are abnormally slow and have a greatly extended duration.

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FilenameFormatSizeDescription
GBB_499_sm_Suppl1a_sib_SLC.avi638KSupporting info item
GBB_499_sm_Suppl1b_mut_SLC.avi601KSupporting info item
GBB_499_sm_Suppl2a_sib_OBend.avi1090KSupporting info item
GBB_499_sm_Suppl2b_mut_OBend.avi980KSupporting info item

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