Are there differences in brain morphometry between twins and unrelated singletons? A pediatric MRI study


  • S. J. Ordaz,

    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
    2. Department of Psychology, University of Pittsburgh, Pittsburgh, PA, USA
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  • R. K. Lenroot,

    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
    2. University of New South Wales and Prince of Wales Medical Research Institute, Sydney, Australia
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  • G. L. Wallace,

    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
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  • L. S. Clasen,

    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
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  • J. D. Blumenthal,

    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
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  • J. E. Schmitt,

    1. Virginia Institute for Psychiatric and Behavioral Genetics and Departments of Psychiatry and Human Genetics, Virginia Commonwealth University, Richmond, VA, USA
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  • J. N. Giedd

    Corresponding author
    1. Child Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Department of Health and Human Services, Bethesda, MD
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J. N. Giedd, MD, Building 10, Room 4C110, 10 Center Drive, Child Psychiatry Branch, National Institute of Mental Health, Bethesda, MD 20892, USA.


Twins provide a unique capacity to explore relative genetic and environmental contributions to brain development, but results are applicable to non-twin populations only to the extent that twin and singleton brains are alike. A reason to suspect differences is that as a group twins are more likely than singletons to experience adverse prenatal and perinatal events that may affect brain development. We sought to assess whether this increased risk leads to differences in child or adolescent brain anatomy in twins who do not experience behavioral or neurological sequelae during the perinatal period. Brain MRI scans of 185 healthy pediatric twins (mean age = 11.0, SD = 3.6) were compared to scans of 167 age- and sex-matched unrelated singletons on brain structures measured, which included gray and white matter lobar volumes, ventricular volume, and area of the corpus callosum. There were no significant differences between groups for any structure, despite sufficient power for low type II (i.e. false negative) error. The implications of these results are twofold: (1) within this age range and for these measures, it is appropriate to include healthy twins in studies of typical brain development, and (2) findings regarding heritability of brain structures obtained from twin studies can be generalized to non-twin populations.

The study of brain development in twins allows exploration of the relative contributions of genetic and environmental influences to variations in brain structure (e.g. Baare et al. 2001; Brun et al. 2009; Hulshoff Pol et al. 2006; Lenroot et al. 2009; Peper et al. 2009; Schmitt et al. 2007, 2008; Thompson et al. 2001; Wallace et al. 2006; Wright et al. 2002). The generalizability of these studies rests on the assumption that brain structure in twins is comparable to that of singletons. However, twins are faced with additional challenges during early development. In the intrauterine environment each twin must compete against the other for limited space and nutritional resources that are fully available to a singleton fetus. Typically developing twins have shorter gestational ages, lower weights given their gestational age (Buckler & Green 2004; Glinianaia et al. 2000; Liu & Blair 2002; Powers & Kiely 1994) and increased risk of perinatal complications (Rao et al. 2004). Studies of preterm, low birth weight singletons have shown that such early developmental disturbances can lead to later reductions in cortical and subcortical brain volumes in childhood (Peterson et al. 2000). It is not known whether the additional stresses of an uncomplicated twin pregnancy have similar adverse effects on the structural development of the brain during childhood and adolescence.

One previous study has been published comparing brain anatomy between twins and their healthy siblings. This study was performed in an adult population and reported a significant difference in cerebral white matter volume that was no longer significant after correcting for twins' smaller intracranial volumes; importantly, they reported no differences in total brain volume (Hulshoff Pol et al. 2002). However, it is possible that the effects of adverse events occurring in the pre- or perinatal periods gradually diminish, such that differences that are still significant and observable during childhood (Buckler & Green 2004; Wilson 1979) are no longer perceptible in adulthood. A longitudinal study of head circumference found that twins had smaller head circumferences that persisted from birth through age 4 (Buckler & Green 2004), and a longitudinal study found that twins had lower IQs than singletons at age 4, but were no longer different by age 6 (Wilson 1974). Additionally, a study of physical growth in twins and singletons reported that twins reach parity with singletons in height and weight by late childhood (Wilson 1979). Thus, pediatric studies using other metrics of brain and cognitive development suggest that by adulthood twins may catch up to singletons, but to date no known studies have examined differences in brain morphometry in pediatric twins and singletons. Determining the comparability of twin and singleton brain development in younger ages is necessary to determine whether results of twin studies in pediatric populations can be generalized to non-twin populations and to establish whether individuals born as twins can be included routinely with singletons in studies of typical brain development.

We therefore compared measures of brain anatomy, including total brain volume, lobar volumes and area of the corpus callosum, between pediatric twins and unrelated singleton participants. Although scans of siblings of some of the twin subjects have been acquired, we chose to compare to unrelated singletons because of the availability of a much larger sample size and better matching for age and gender which are best suited for the objectives of this study.

Materials and methods


Healthy child and adolescent twins and singletons were recruited for participation in an ongoing longitudinal pediatric brain magnetic resonance imaging (MRI) study at the Child Psychiatry Branch of the National Institute of Mental Health (NIMH) (Giedd et al. 2009). Singletons were recruited locally and twins locally and nationally. Parents of prospective participants were interviewed by phone and asked to report their child's health, developmental and educational histories.

Participants were excluded if they had ever taken psychiatric medications, received psychiatric diagnoses, or had any other trauma or condition known to affect gross brain development. Twins diagnosed with twin-to-twin transfusion syndrome during gestation were excluded from the study. Inclusion criteria for both twins and singletons included a minimum gestational age of 29 weeks and a minimum birth weight of 1500 g. These ranges were chosen to accommodate the typically shorter gestational ages and lower birth weights of twins, yet to exclude neonates whose circumstances are extraordinary and place them at higher risk for adverse neurodevelopmental outcomes (Nagy 2003; Peterson et al. 2000; Reiss et al. 2004). Approximately 80% of families responding to advertisements met study criteria.

The NIMH Institutional Review Board approved the protocol. Written informed assent was obtained from all participants under the age of 18 in addition to consent from parents/guardians; individuals 18 years and older provided written informed consent. All subjects were administered the Wechsler Abbreviated Scale of Intelligence (WASI) (Wechsler 1999) or, for those participants younger than 7 years of age, the Vocabulary, Similarities, Information, Block Design, Matrix Reasoning, Picture Concepts, and Coding subtests of the Wechsler Preschool and Primary Scale of Intelligence, Third Edition (WPPSI-III) (Wechsler 2002). Using these instruments, Full Scale IQ as well as Verbal and Performance IQ were calculated. Socioeconomic status (SES) was determined using the Hollingshead SES scale (Hollingshead & Redlich 1958, 2007). Handedness was obtained using the Physical and Neurological Examination for Soft Signs (PANESS) (Denckla 1985). Birth data were obtained by parental report. Zygosity of the twins was determined by DNA analysis of buccal cheek swabs using 9–21 unlinked short tandem repeat loci for a minimum certainty of 99% by BRT Laboratories, Inc. (Baltimore, MD).

Scan acquisition

All images were acquired on the same General Electric 1.5 Tesla Signa Scanner located at the NIH Clinical Center in Bethesda, Maryland. A three-dimensional spoiled gradient recalled echo sequence in the steady state, designed to optimize discrimination between gray matter, white matter and CSF, was used to acquire 124 contiguous 1.5 mm thick slices in the axial plane (TE /TR = 5/24; flip angle = 45 degrees, matrix = 256 × 192, NEX = 1, FOV = 24 cm , acquisition time 9.9 min). A Fast Spin Echo/Proton Density weighted imaging sequence was also acquired for clinical evaluation.

Image analysis

The native MRI scans were registered into standardized stereotaxic space using a linear transformation (Collins et al. 1994) and corrected for non-uniformity artifacts (Sled et al. 1998). The registered and corrected volumes were segmented into white matter, gray matter and cerebrospinal fluid using a neural net classifier (Zijdenbos et al. 2002). Region of interest analysis was performed by combining tissue classification information with a probabilistic atlas (Collins et al. 1995). The regional volumes which have shown high agreement with conventional hand tracing measures and are included in this analysis are the right-sided, left-sided and total volumes of the following regions: total cerebrum (which is the sum of the total gray matter and total white matter), total gray matter, total white matter, gray and white matter of the frontal, temporal, parietal and occipital lobes, as well as the caudate nucleus. A validation study comparing this method with manual segmentation found volumetric differences to be less than 10% and volumetric overlap to be greater than 85% (Collins et al. 1995). An independent validation study of caudate volumes comparing a sample of manually defined caudate volumes from 263 pediatric subjects from this laboratory with automated measures found them to be highly correlated (Spearman's rho >0.72, P < 0.01 for left, right and total caudate volumes). Midsagittal area of the corpus callosum was quantified as per a previously described protocol (Giedd et al. 1999). Scans were reviewed by a trained rater (J.B.), and those with gross motion artifact were removed from the sample.

Statistical analysis

One twin each from 185 twin pairs was randomly selected for analysis using a computerized random selection algorithm. A set of 167 unrelated singletons was selected from the larger singleton study group matched by sex and age at scan (see Table 1). Data were screened for outliers, and none were removed for purposes of the analysis. Chi-square tests and analysis of variance (anova) were used to determine group differences between twins and singletons for right, left and total sizes of all brain regions.

Table 1.  Demographic data
VariableSingleton mean (SD)Twin mean (SD)Twin/Singleton comparison
Test statisticP
  1. Higher values for the SES variable indicate lower socioeconomic status. **Significant at the P < 0.01 level.

Age11.0 (3.6)10.9 (3.6)F(1,350) = 0.130.716
Sex (% male)52.7%53.5%χ2(1, N = 352) = 0.020.878
Race/Ethnicity (% non-Hispanic Caucasian)77.8%93.0%χ2(1, N = 352) = 16.480.000**
Handedness (% right-handed)91.6%84.2%χ2(2, N = 350) = 5.770.056
SES37.7 (19.9)43.3 (17.5)F(1, 335) = 7.460.007**
Full Scale IQ115.8 (13.4)110.3 (12.5)F(1, 339) = 15.450.000**
Verbal IQ114.5 (12.6)109.7 (13.2)F(1, 265) = 7.660.006**
Performance IQ113.7 (12.2)108.7 (13.4)F(1, 266) = 8.630.004**
Birth weight (ounces)122.8 (17.6)94.0 (18.6)F(1, 286) = 161.620.000**
Gestational age (weeks)39.7 (1.8)37.0 (2.5)F(1, 252) = 33.690.000**

ancova was used to estimate potential effects of IQ and SES differences on comparison of brain measures between twins and singletons. For IQ, the assumption of homogeneity of regression for ancova was violated in the following regions: total cerebral volume, total gray matter, total white matter, frontal gray matter, temporal white matter, occipital gray matter, occipital white matter and the midsagittal corpus collosum. For these regions, we addressed this violation by following the recommendations of Tabachnick and Fidell (2001) and Keppel et al. 2004, transforming IQ into a blocking variable that was then entered as a factor and an interaction factor within a regression framework. To create the blocking variable, individuals were divided into three equally sized IQ groups (low, medium, high). In all other brain regions which did not violate this assumption, ancova was run using a continuous IQ covariate because it has better precision and power. As SES is not a parametric covariate, SES scores were also arbitrarily divided into three similar sized groups and effects on outcome measures predicted using regression. To control for type I error, a false discovery rate procedure (Benjamini & Hochberg 1995) was applied to each of the three sets of group comparisons. In order to explore whether group differences in brain volume change with age (given postulations that twin brain growth may catch up during the postnatal period), we tested the interaction of group by age in all of the aforementioned brain regions using a continuous measure of age.

Effect sizes were estimated by calculating d = (µ1−µ2)diff (Cohen 1992), where σdiff is the standard deviation of the difference in means between the two groups. Cohen's conventions for the magnitude of effect size were utilized; effect sizes were defined as ‘small’ if d≤ 0.2, ‘medium’ if 0.2 < d < 0.8, and ‘large’ if d≥ 0.8. Given concern for type II error (i.e. false negative) for this hypothesis, post-hoc criterion power analyses were used to compute the alpha level that would be compatible with a low type II error (β = 0.05; 1 −β = 0.95) for a sample of this size and for small effect sizes. Analyses were performed using spss 14.0. Sample sizes needed to obtain power were calculated using the g-Power 3.0 software (Faul et al. 2007).



Subjects ranged in age from 4.6 to 19.5 years. Means, standard deviations, test statistics and P-values for all demographic data comparisons are shown in Table 1. There were no significant differences between twins and singletons in age or ratio of males to females. The twin sample was composed of a significantly larger percentage of non-Hispanic Caucasian participants (93.0% non-Hispanic Caucasian in twins versus 77.8% in singletons). There were significant group differences on SES as well as Full Scale IQ, Verbal IQ and Performance IQ scores; twins were lower SES and had lower IQ scores. Handedness differences were not significant, but twins trended toward having a higher percentage of mixed or left-handed subjects than did singletons.

Zygosity tests indicated that 123 (66.4%) twins were from monozygotic (MZ) pairs and 58 (31.4%) were from same-sex dizygotic (DZ) pairs. Four individuals (2.2%) from same-sex pairs did not complete a zygosity test. Of the twins randomly selected for these analyses, 95 (51.4%) were first-born twins, 85 (45.9%) were second-born twins and the birth order of 5 (2.7%) was unreported. The distribution of males and females did not differ within zygosity subgroups (χ2(2) = 0.02, not significant (n.s.)) nor birth order subgroups (χ2(1) = 0.04, n.s.).

Birth history variables

An examination of birth history including means, standard deviations, F-values and P-values for comparisons of birth weight and gestational age are shown in Table 1. Consistent with previous reports, twins had lower mean birth weights and younger gestational ages compared to singletons.

Volumetric brain measurements

Means, standard deviations, F-values, P-values and effect sizes for all comparisons of total volumetric brain measurements are shown in Table 2 and comparisons broken down by laterality are shown in Table 3. Following application of the false discovery rate procedure, no significant differences were found for any of the regions analyzed. Cohen's d effect size reached a maximum absolute value of 0.074. Significance for the right and left hemispheres in each brain structure followed the same pattern as for each brain structure as a whole. As the lateralized and combined hemisphere results are comparable, for clarity of presentation, analyses are henceforth presented for each given structure as a whole.

Table 2.  Volumetric brain data
Brain regionSingleton mean (SD) in cubic cmTwin mean (SD) in cubic cmTwin/Singleton comparison*Twin/Singleton comparison controlling for IQTwin/Singleton comparison controlling for SESGroup × Age interaction
F(1,350)PCohen's dTest statisticPEffect sizet(331)PPr2t(348)P
  1. *Statistics reflect twin/singleton comparisons that are not corrected for IQ or SES.

  2. Negative value indicates smaller twin volume.

  3. Effect sizes are reported as partial eta squared (η2) for regions in which a continuous covariate was used and as a partial correlation (Pr2) for regions in which a categorical covariate was used.

  4. §n.s. after false discovery rate correction.

Total cerebral volume1162.7 ( 121.2)1164.6 ( 109.6)0.020.880.003t(335) = 0.870.39Pr2 = 0.0020.240.810.0001.250.21
Total gray matter738.7 ( 77.8)738.4 ( 66.7)0.000.97−0.039t(335) = 1.000.32Pr2 = 0.0030.060.950.0001.020.31
Total white matter424.0 ( 57.1)426.2 ( 54.6)0.130.72−0.017t(335) = 0.610.55Pr2 = 0.0010.410.690.0001.270.21
Frontal gray matter234.3 ( 25.0)233.3 ( 22.0)0.150.700.042t(335) = 0.160.88Pr2 = 0.000−0.320.750.0000.910.37
Frontal white matter163.0 ( 22.6)163.6 ( 21.9)0.070.78−0.029F(1,338) = 0.680.41η2 = 0.0020.400.690.0000.970.33
Parietal gray matter125.9 ( 15.6)125.0 ( 13.4)0.300.590.058F(1,338) = 0.010.91η2 = 0.000−1.740.080.0091.390.17
Parietal white matter82.6 ( 11.9)82.7 ( 11.6)0.000.96−0.006F(1,338) = 0.650.42η2 = 0.0020.070.950.0000.830.41
Temporal gray matter189.5 ( 20.2)189.8 ( 18.2)0.020.88−0.017F(1,338) = 1.100.30η2 = 0.0030.430.670.0010.920.36
Temporal white matter91.4 ( 13.1)91.7 ( 12.2)0.040.85−0.021t(335) = 0.970.33Pr2 = 0.0030.200.840.0000.730.47
Occipital gray matter65.1 ( 10.2)64.8 ( 9.1)0.010.760.033t(335) = 0.410.68Pr2 = 0.0000.330.740.0001.790.08
Occipital white matter40.4 ( 6.7)40.9 ( 6.9)0.450.51−0.072t(335) = 1.290.20Pr2 = 0.0051.120.260.0041.070.29
Lateral ventricles10.2 ( 6.1)10.7 ( 6.0)0.490.49−0.074F(1,338) = 0.290.75η2 = 0.0000.630.530.001−2.300.02§
Caudate nucleus9.9 ( 1.0)9.9 ( 1.1)0.000.980.010F(1,338) = 0.280.60η2 = 0.001−1.640.100.0080.980.33
Midsagittal corpus callosum (area in cm2)527.0 ( 78.1)530.9 ( 73.2)0.230.63−0.051t(335) = 1.010.31Pr2 = 0.0030.330.750.000−0.730.94
Table 3.  Lateralized volumetric brain data
Brain regionTwin mean (SD) in cubic cmSingleton mean (SD) in cubic cmTwin/Singleton comparison*Twin/Singleton comparison controlling for IQTwin/Singleton comparison controlling for SESGroup × Age interaction
F(1,350)PCohen's dTest statisticPEffect sizet(331)PPr2t(348)P
  1. *Statistics reflect twin/singleton comparisons that are not corrected for IQ or SES.

  2. Negative value indicates smaller twin volume.

  3. Effect sizes are reported as partial eta squared (η2) for regions in which a continuous covariate was used and as a partial correlation (Pr2) for regions in which a categorical covariate was used.

  4. §n.s. after false discovery rate correction.

Frontal gray matter—left117.9 ( 12.5)117.2 ( 11.1)0.350.560.063F(1, 338) = 0.000.96η2 = 0.000−0.500.620.0050.870.38
Frontal gray matter—right116.3 ( 12.6)116.1 ( 11.2)0.040.850.022F(1, 338) = 0.220.64η2 = 0.001−0.130.900.0150.930.35
Frontal white matter—left81.6 ( 11.3)81.4 ( 11.0)0.050.820.023F(1, 338) = 0.130.72η2 = 0.000−0.090.930.0000.910.36
Frontal white matter—right81.4 ( 11.4)82.3 ( 11.0)0.590.44−0.082F(1, 338) = 1.650.20η2 = 0.0050.890.370.0020.670.50
Parietal gray matter—left62.4 ( 8.0)61.7 ( 6.8)0.730.400.091F(1, 338) = 0.020.90η2 = 0.000−1.030.310.0030.990.32
Parietal gray matter—right63.5 ( 8.0)63.3 ( 6.9)0.050.830.023F(1, 338) = 0.130.72η2 = 0.000−0.660.510.0011.730.09
Parietal white matter—left40.7 ( 6.0)40.9 ( 5.8)0.060.81−0.026t(335) = 1.020.31Pr2 = 0.0030.280.780.0000.910.36
Parietal white matter—right41.9 ( 6.1)41.8 ( 6.0)0.020.900.014F(1, 338) = 0.260.61η2 = 0.001−0.150.880.0000.730.47
Temporal gray matter—left93.2 ( 9.9)93.2 ( 9.0)0.120.730.004t(335) = 0.790.43Pr2 = 0.0020.320.750.0001.070.29
Temporal gray matter—right96.3 ( 10.4)96.7 ( 9.4)0.000.97−0.037F(1, 338) = 1.560.21η2 = 0.0050.520.610.0010.760.45
Temporal white matter—left45.1 ( 6.6)44.9 ( 6.0)0.100.750.033t(335) = 0.470.64Pr2 = 0.001−0.250.800.0000.690.49
Temporal white matter—right46.3 ( 6.6)46.8 ( 6.3)0.460.50−0.072t(335) = 1.430.15Pr2 = 0.0060.630.530.0010.760.45
Occipital gray matter—left33.9 ( 5.3)33.6 ( 5.0)0.260.610.054F(1, 338) = 0.120.73η2 = 0.0000.120.900.0001.490.14
Occipital gray matter—right31.2 ( 5.2)31.2 ( 4.6)0.010.940.009t(335) = 0.560.57Pr2 = 0.0010.520.610.0011.950.05
Occipital white matter—left20.1 ( 3.4)20.9 ( 3.6)5.500.02§−0.251t(335) = 2.880.00§Pr2 = 0.0242.770.01§0.0231.190.23
Occipital white matter—right20.3 ( 3.5)19.9 ( 3.6)1.080.300.111t(335) = −0.390.70Pr2 = 0.000−0.590.560.0010.850.40
Lateral Ventricles—left5.3 ( 3.3)5.5 ( 3.3)0.330.56−0.062F(1, 338) = 0.060.80η2 = 0.0000.410.690.000−2.590.01§
Lateral Ventricles—right5.0 ( 3.1)5.2 ( 3.1)0.560.45−0.080F(1, 338) = 0.260.61η2 = 0.0010.800.420.002−1.730.09
Caudate Nucleus—left5.0 ( 0.5)5.0 ( 0.5)0.450.50−0.070F(1, 338) = 1.590.21η2 = 0.0050.700.480.0021.120.26
Caudate Nucleus—right4.9 ( 0.5)4.9 ( 0.5)0.540.460.078F(1, 338) = 0.060.81η2 = 0.000−0.520.600.0010.810.42

Due to significant group differences in IQ and SES, brain volume comparisons were repeated with each of these variables added as covariates. IQ and SES accounted for overlapping variance (Spearman's rho correlation coefficient = −0.324; P < 0.001); separate analyses were therefore run for each of these two covariates as described in the Materials and Methods. An ancova was used to covary for IQ, using a continuous measure of IQ, except in regions in which the assumption of homogeneity of regression for ancova was violated (total cerebral volume, total gray and white matter, frontal gray matter, temporal white matter, occipital gray matter, occipital white matter and midsagittal corpus collosum). In these regions we used a categorical IQ variable as a blocking variable as described in the Section Materials and Methods. There were no significant differences in brain volume between twins and singletons when covarying IQ as a continuous (F(1,338), all Ps ≥ 0.298, see Tables 2 and 3) or categorical variable (t(335), all Ps ≥ 0.197). A categorical measure was used to covary for SES given that it was not a parametric covariate. Comparisons using this covariate indicated no significant effects of SES on twin–singleton comparisons for any region (t(331), all Ps ≥ 0.083, see Tables 2 and 3).

To examine whether group differences decreased with age, group by age interactions were tested in a regression framework using a continuous measure of age. Results indicated no significant interactions in any of the regions measured after application of the false discovery rate procedure (t(348), all Ps ≥ 0.022, see Tables 2 and 3).

Cohen's d effect sizes for the uncorrected twin/singleton comparisons were all small in magnitude, ranging from 0.003 to 0.074 for volumetric brain data and from 0.004 to 0.251 for lateralized volumetric brain data (see Tables 2 and 3). Post-hoc criterion power analysis to determine a significance level α, which is compatible with a low type II error (β = 0.05), produced a high alpha error probability (α = 0.410). Even if this significance criterion was used in order to minimize type II error, there would be no significant differences in any brain region after correcting for multiple comparisons.


We found no significant differences between pediatric twins and singletons for total and lobar gray and white matter volumes, caudate nucleus volumes, ventricular volumes or area of the corpus callosum. Effect sizes of twin–singleton differences were small (Cohen 1992), suggesting a high degree of overlap in the distributions of each group's brain volumes regardless of the region assessed. Post-hoc power analyses confirmed that the sample had sufficient power to make a type II error unlikely. These results support the generalizability of findings obtained from studies of brain development in twins within this age range to non-twin populations, and the use of twins as subjects in studies of typical brain development.

In the one previous study comparing 112 adult twins and 34 of their healthy non-twin siblings (Hulshoff Pol et al. 2002), the only difference found was in white matter volume, which was reported to be smaller in the adult twins, and no longer significant after controlling for group differences in intracranial volume. Comparison between studies is limited by differences in study design, including adult versus pediatric populations, methods of image acquisition and methods of image analysis. In addition, the present study compared twins to unrelated singletons whereas Hulshoff Pol et al. (2002) used twin siblings as a comparison group. Nonetheless, it is possible that white matter differences only emerge after volumetric increases due to myelination during childhood and adolescence, raising the question of whether growth trajectories may be different in twins and singletons.

Our findings indicate that age does not moderate differences in twin and singleton brain volumes between ages 4 and 19. That is, brain volumes do not ‘catch up’ over the course of childhood and adolescence. However, studies comparing growth trajectories of height, weight and head circumference between twins and singletons have found most pronounced differences at birth that rapidly diminish due to catch-up growth in twins (Buckler & Green 2004; Wilson 1979). Together with this research, our results indicate that catch-up growth, if it occurs at all, is complete by early childhood. Research comparing cognitive development between twins and singletons has produced mixed results. Studies using data acquired from children in the 1940s and 1950s found twins have IQs that are on average five points lower than IQs of singletons (Deary et al. 2005; Drillien 1961; Mehrotra & Maxwell 1949; Record et al. 1970; Santon 1957). A subsequent study (Wilson 1974) found an IQ difference among four and five year olds that was not present among six year olds, suggesting differences may be due to a temporary developmental lag in twins. Recent studies of adolescents (Christensen et al. 2006) and adults (Posthuma et al. 2000) found no difference in cognitive performance in twins compared to singletons. Studies of heritability of intelligence have found that IQ becomes increasingly heritable with age, suggesting that cognitive abilities may also have some resilience to early adverse environmental influences (McClearn et al. 1997; Plomin & Kosslyn 2001; Plomin et al. 1994). Within our sample, IQ was approximately five points lower in twins than in singletons. However, interpretation of this finding is complicated by the significantly lower SES of twins in our sample, likely due to ascertainment bias.

The present study possesses several limitations. The twins included in this study were not chosen to be representative of the twin population as a whole, but rather representative of twins who are likely to meet typical screening criteria as subjects for studies of normal brain development. Both twins and singletons with a history of severe adverse birth events, atypically short gestational age (less than 29 weeks) or very low birth weight (less than 1500 g) were excluded from the present study. These risk factors have been shown to affect brain development into childhood and adolescence and are more likely to occur in twins (Parker et al. 2008; Peterson et al. 2000). Twins (and group-matched singletons) from our study had higher than average IQ scores, which may limit generalizability to the twin and singleton populations. It should be noted that a goal of this study was to explore whether results from twin studies of brain morphometry can be related to comparable research conducted in singletons. As many published studies of brain development in twins (Lenroot et al. 2009; Schmitt et al. 2007, 2008; Wallace et al. 2006) and singletons (Lenroot et al. 2007; Lu 2009; Paus et al. 1999; Reiss et al. 1996; Shaw et al. 2006) have used samples with similarly high IQ scores (when these scores are obtained and reported), we believe that the participants in this study are representative of individuals who volunteer and meet screening criteria for imaging studies in general. It should be noted that the youngest participants in the current study were four years old, and that results should not be taken as indicative of findings in infants and very young children as previously mentioned. Furthermore, the current study is cross-sectional; future studies using longitudinal data will allow determination of whether developmental trajectories differ between twins and singletons. Finally, our conclusions can only be held as valid for the structures reported. Studies using higher resolution techniques such as voxel-based measurements of cortical features may detect more subtle twin–singleton differences.

In summary, this study demonstrates that brain lobar volumes, ventricular size and area of the corpus callosum are not different between twins and singletons during childhood and adolescence. This supports the utility of brain morphometric data obtained from twins during childhood and adolescence in studies of healthy brain development, and the external validity of large-scale twin studies in exploring genetic and environmental sources of variation in brain development.


This work was supported by the Intramural Research Program of the NIH and by National Institute of Mental Health (NIH grants MH-20030 and MH-65322). The authors would like to thank the participants and their families for their time, and also Elizabeth Molloy, Michael Rosenthal, Blythe Rose and Kristin Taylor for their assistance with data collection.