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Figure S1: Principal component analysis of behavioral measures. To determine the number of clusters to utilize for K-means clustering, a principal component analysis was performed. Eigenvalues are shown with the corresponding percent and cumulative percent variance explained. We based the number of clusters on the number of eigenvalues that explain approximately 75% of the total variance of the model.

Figure S2: Use of K-means clustering to depict changes in behaviors resulting from mutations (females). Red represents an increase in mutant relative to wild type, green represents a decrease and black signifies no difference between mutant and wild type. Behaviors and genotypes, which are significantly related, are shown as blocks or clusters. Data are from female mice; results from males are shown in Fig. 1. Details on mutations and behaviors are given in Tables S1 and S2. See Methods and materials for K-means clustering details.

Figure S3: Relationships among two-bottle choice drinking and other behaviors for wild-type (upper panel) and mutant (lower panel) mice (females). Significant correlations are noted by the arrows and connecting lines with the coefficient of determination (r2) given on each line; dotted lines represent positive correlations and solid lines are negative correlations. Data are from female mice; results from males are in Fig. 3. Abbreviations used in Figs. 2–5 and Figs. S2–S5 are as follows: 2BAE, two-bottle choice, amount of alcohol consumed denoted by the concentration of alcohol (2BAE12 is amount of 12% solution consumed); AWN, alcohol withdrawal negative, acute anticonvulsant measure; AWP, alcohol withdrawal positive, acute alcohol withdrawal severity; CTA, conditioned taste aversion; DID, drinking in the dark or limited access drinking; LORR, duration of loss of righting reflex produced by ethanol; NOVELTY, motor response to a novel situation; SAC1/SAC2, preference for 0.033/0.066% saccharin; SHAC, sustained high alcohol consumption; QN1/2, preference for 0.03/0.06 mm quinine.

Figure S4: Relationships among limited access drinking (DID) and other behaviors for wild-type (upper panel) and mutant (lower panel) mice (females). Significant correlations are noted by the arrows and connecting lines with the coefficient of determination (r2) given on each line; dotted lines represent positive correlations and solid lines are negative correlations. Data are from female mice; results from males are in Fig. 4.

Figure S5: Relationships among limited access (SHAC) drinking and other behaviors for wild-type (upper panel) and mutant (lower panel) mice (females). Significant correlations are noted by the arrows and connecting lines with the coefficient of determination (r2) given on each line; dotted lines represent positive correlations and solid lines are negative correlations. Data are from female mice; results from males are in Fig. 5.

Table S1: Summary of mutant mice.

Table S2: Summary of raw data used for correlational analyses in male mice.

Table S3: Summary of raw data used for correlational analyses in female mice.

Table S4: Correlational analyses of behaviors measured in wild-type male mice. Significant correlations are in bold. Symbols are defined in Table S2.

Table S5: Correlational analyses of behaviors measured in mutant male mice. Significant correlations are in bold. Symbols are defined in Table S2.

Table S6: Correlational analyses of behaviors measured in wild-type female mice. Significant correlations are in bold. Symbols are defined in Table S2.

Table S7: Correlational analyses of behaviors measured in mutant female mice. Significant correlations are in bold. Symbols are defined in Table S2.

Table S8: Correlational analyses of behaviors measured in wild-type male mice. Data include DID ethanol consumption and are thus limited to the 19 mutants tested for DID. Significant correlations are in bold. Symbols are defined in Table S2.

Table S9: Correlational analyses of behaviors measured in mutant male mice. Data include DID ethanol consumption and are thus limited to the 19 mutants tested for DID. Significant correlations are in bold. Symbols are defined in Table S2.

Table S10: Correlational analyses of behaviors measured in wild-type female mice. Data include DID ethanol consumption and are thus limited to the 19 mutants tested for DID. Significant correlations are in bold. Symbols are defined in Table S2.

Table S11: Correlational analyses of behaviors measured in mutant female mice. Data include DID ethanol consumption and are thus limited to the 19 mutants tested for DID. Significant correlations are in bold. Symbols are defined in Table S2.

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GBB_780_sm_TableS3.xls322KSupporting info item
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