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gbb848-sup-0001-FigureS1.epsPS document449K Figure S1: Animals show similar levels of exploration in the NOR test. (a) SPARC genotype did not affect the number of visits to either object in the habituation session 1, and (b) all three objects were visited a similar number of times. (c) SPARC genotype did not affect the amount of time animals spent exploring each object in the habituation session 1, but (d) animals spent less time exploring object A. *P < 0.05 vs. object A. (e) Animals show similar levels of locomotion during the habituation sessions and in the novel object session, but KO mice show reduced locomotion during the spatial novelty session. *P < 0.05 vs. WT. Mean ± SEM. N = 15 per group.
gbb848-sup-0002-FigureS2.epsPS document10494K Figure S2: Representative images for c-Fos and neurogenesis analysis. (a) Representative image of the DG of a KO mouse, processed for immunohistochemistry anti-c-Fos, after a footshock protocol. c-Fos-positive cells in the granular cell layer (arrow head) were counted under × 400 magnification in a light-field microscope. Low magnification images were obtained to determine the area of the granular cell layer (delimited by a yellow line in the figure). ML, molecular layer. Scale bar = 0.25 mm. (b) Representative image obtained after double immunofluorescence anti-BrdU and DCX, for a Het mouse. To determine the percentage of BrdU-positive cells, we obtained × 400 confocal images spanning the ‘z’ axis and we counted the total of BrdU-positive cells and double-labeled cells. Examples of BrdU-positive, DCX-positive and double-labeled cells are shown. GCL, granular cell layer. Scale bar = 50 µm.
gbb848-sup-0003-FigureS3.epsPS document400K Figure S3: Expression of SPARC in the DG does not rescue the increase in anxiety-related behaviors observed in SPARC KO mice. Animals were stereotaxically injected with Adβgal or AdSPARC and tested 2 weeks after (a). AdSPARC- and Adβgal-injected mice spend a similar amount of time (b) and a similar percentage of distance (c) in the center of the open field. Both treatment groups spend the same time in the lit compartment (D) in the light/dark test. Mean ± SEM. N = 10 per group.

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