• detergent;
  • MTT;
  • oral mucosa;
  • toothpaste;
  • viability

Abstract:  Objectives:  Toothpastes are thought to be of benefit to cleaning teeth but may also have the potential for soft tissue damage at least on the cellular level by inclusion of detergents in their formulation. The aim of this study was to observe the in vitro response of oral mucosa like cells to toothpaste detergents.

Methods:  TERT-1 keratinocytes were exposed to varying concentrations of the detergents Adinol, Sodium Lauryl Sulphate, Tego Betain and Pluronic as well as PBS and culture medium. After 2-min exposure, cells were washed and incubated in fresh medium for 24 h. Cell death was then spectrophotmetrically measured using an MTT assay.

Results:  Except for Pluronic, cell viability was markedly reduced for all detergents at all increasing concentrations when compared to the positive medium control. Cells treated with Pluronic were stimulated compared to medium alone.

Conclusions:  These in vitro data suggest that some detergents may have the potential to cause soft tissue damage in the mouth. Although in vivo, saliva may neutralize such effects. The results for Pluronic suggest a possible oxidative stress response that bears further study.