• Open Access

Molecular Analysis of an XY Mare with Gonadal Dysgenesis


E. P. Cribiu, INRA, Laboratoire de Génétique Biochimique et de Cytogénétique, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josus CEDEX, France


In this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome.