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The “Epidermophyton factor” (EPF) was formed best on cultivation in medium where the reaction was maintained alkaline. The substance gave the chemical colour reactions expected of a steroid. In these reactions and in comparative chromatography EPF closely resembled fucidin, but it was found to be a less acid substance. Nor was EPF identical with 3-keto-fusidic acid, helvolic acid or cephalosporin P1.