Gas Chromatography of Bacterial Whole Cell Methanolysates

I. The Usefulness of Trimethylsilyl- and Trifluoroacetyl Derivatives for Strain and Species Characterization

Authors

  • Erik Jantzen,

    1. Statens Institutt for Folkehelse, Oslo, Norway, and Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Oslo, Norway
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  • Leif Oddvar Frøholm,

    1. Statens Institutt for Folkehelse, Oslo, Norway, and Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Oslo, Norway
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  • Ruth Hytta,

    1. Statens Institutt for Folkehelse, Oslo, Norway, and Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Oslo, Norway
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  • Kjell Bøvre

    1. Statens Institutt for Folkehelse, Oslo, Norway, and Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Oslo, Norway
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Abstract

Gas-liquid chromatography (GLC) of trifluoroacetylated (TFA) whole cell methanolysates as a procedure for obtaining species specific GLC elution profiles has been introduced. This method and a modification of the previously described trimethylsilyl (TMS) derivatization used for identical purpose were tested on four strains belonging to the bacterial species Neisseria catarrhalis, N. ovis and N. meningitidis. The TFA and TMS elution profiles are compared, and their usefulness in characterization of strains and species in relation to the best reproducibility obtainable is discussed. The TFA method gives better resolved and more reproducible elution profiles. The method appears to have a high potential as an aid in bacterial classification and identification.

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