Studies of a Li-Hapten Isolated from Cell-Walls of the Rough Mutant Salmonella Typhimurium 395 MR10

Authors

  • A. A. Lindberg,

    Corresponding author
    1. Department of Bacteriology, Statens Bakteriologiska Laboratorium, Stockholm, Sweden
    2. Department of Bacteriology, Karolinska institutet, Stockholm
    3. Department of Organic Chemistry, Stockholms universitet, Stockholm
      Statens Bakteriologiska Laboratorium, S-105 21 Stockholm, Sweden.
    Search for more papers by this author
  • T. Holme,

    1. Department of Bacteriology, Statens Bakteriologiska Laboratorium, Stockholm, Sweden
    2. Department of Bacteriology, Karolinska institutet, Stockholm
    3. Department of Organic Chemistry, Stockholms universitet, Stockholm
    Search for more papers by this author
  • C. G. Hellerqvist,

    1. Department of Bacteriology, Statens Bakteriologiska Laboratorium, Stockholm, Sweden
    2. Department of Bacteriology, Karolinska institutet, Stockholm
    3. Department of Organic Chemistry, Stockholms universitet, Stockholm
    Search for more papers by this author
  • S. Svensson

    1. Department of Bacteriology, Statens Bakteriologiska Laboratorium, Stockholm, Sweden
    2. Department of Bacteriology, Karolinska institutet, Stockholm
    3. Department of Organic Chemistry, Stockholms universitet, Stockholm
    Search for more papers by this author

Statens Bakteriologiska Laboratorium, S-105 21 Stockholm, Sweden.

Abstract

An O-haptenic material (LI-fraction) was isolated from cytoplasmic and cell-wall preparations of the rough mutant Salmonella typhimurium 395 MR10. The yield from the cell-wall preparation, virtually free from cytoplasmic contamination, was approximately ten times higher than that from the cytoplasmic preparation. The LI-fraction obtained from cell-walls was less contaminated with extraneous polysaccharide material. After purification of the LI-fraction with ribonuclease treatment and Sephadex G-100 gel chromatography structural studies using methylation analysis could be performed. The hapten showed the same structural features as the O side-chains of the parent strain S. typhimurium 395 MS. The average number of repeating units in the hapten was 23 as compared to 11 in the O side-chain of the parent strain. Molecular weight determination with Sepharose 6 B gel chromatography revealed that the LI-fraction was polydisperse with a weight average of 26,300 and a number average of 21,800.

Ancillary