5-NUCLEOTIDASE ACTIVITY IN THE HUMAN BREAST

Enzyme-Histochemical Studies

Authors

  • Henning Jensen

    1. The Department of Pathology, Bispebjerg Hospital, Copenhagen, Denmark Heads: Charles Johansen, Erik Bredahl, Aage Johansen, Knud Højgaard
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    • 2

      Nymindevej 13, Vanløse, Copenhagen.


Abstract

Fresh-frozen sections of human breast tissue (210 biopsy specimens) are examined for activity of 5-nucleotidase in various benign and malignant pathological conditions. 5-nucleotidase is found mainly in the fibroblasts and produce a characteristic stroma-response. By nickel blocking of 5-nucleotidase it is possible to distinguish the 5-nucleotidase activity from that of the unspecific alkaline phosphatases. The technique is described. The two enzyme systems are both localized mainly in the fibroblasts, but at different sites.

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