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An indirect immunofluorescence technique using unfixed colonies is applied to human T-mycoplasmas. The method is found to possess the same degree of specificity as the growth-inhibition test and to be well suited for the identification and classification of T-myco-plasmas. The sensitivity is too low for the detection of antibodies in patient sera. A modification of the indirect haemagglutination test applicable to T-mycoplasmas was less specific, but owing to its sensitivity it can be recommended as a supplement to the metabolic-inhibition test in studies of antibodies in patient sera. The polyacrylamide-gel electrophoresis appeared to be unsuitable for the identification and classification of human T-mycoplasma serotypes.