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Keywords:

  • Gram-negative rods;
  • rapid identification;
  • three-tube method;
  • dichotomic key

A three-tube method combined with a dichotomic key is presented which will identify virtually all Enterobacteriaceae as well as a number of other frequently encountered Gram-negative rods at a genus or species level within 20 hours following the primary isolation. The method is shown to be reliable and simple, saving processing time and material.