A SIMPLE TURBIDIMETRIC METHOD FOR SPECIFIC MEASUREMENT OF STAPHYLOCOCCUS AUREUS LIPASE ACTIVITY

Authors


Department of Infectious Diseases, University Hospital, S-221 85 Lund, Sweden.

Abstract

We have developed a simple semiquantitative method for measuring Staphylococcus aureus lipase activity. Enzymatic activity is measured as the decrease in spectrophotometric absorbancy in a lipid emulsion after incubation with the sample. The sensitivity, precision and accuracy are adequate for routine purposes. The assay is rapid and simple and does not require advanced technical equipment.

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