Polymorphism and evolutionary profile of mitochondrial DNA control region inferred from the sequences of Pakistani goats
Version of Record online: 19 JUL 2004
Animal Science Journal
Volume 75, Issue 4, pages 303–309, August 2004
How to Cite
SULTANA, S. and MANNEN, H. (2004), Polymorphism and evolutionary profile of mitochondrial DNA control region inferred from the sequences of Pakistani goats. Animal Science Journal, 75: 303–309. doi: 10.1111/j.1740-0929.2004.00190.x
- Issue online: 19 JUL 2004
- Version of Record online: 19 JUL 2004
- Received 4 December 2003; accepted for publication 19 March 2004.
- displacement loop;
- evolutionary pattern;
- mitochondrial DNA;
- Pakistani domestic goats;
Complete mitochondrial DNA (mtDNA) control region of 30 Pakistani domestic goats was sequenced to investigate the genetic diversity and organization. Twenty-two new haplotypes were observed and all were classified into mt-lineage A. Phylogenetic analysis revealed two distinct clusters in mt-lineage A, A1 and A2. A 17 bp deletion and a 76 bp insertion were detected in the L-domain and observed 10 and one animals, respectively, in 232 Pakistani domestic goats. These remarkable deletion/insertion events would be useful to investigate mtDNA diversity within the highly divergent mt-lineage A. In analysis of the control region feature, the caprine mtDNA was A/T rich as observed for other artiodactyls. Four conserved regions, Block A in the L domain, the C-domain, Block B and CSB1 in the R domain, were defined as well as in the other species.