Characterization of bacteriocin produced by Streptococcus bovis J2 40-2 isolated from traditional fermented milk ‘Dahi’
Article first published online: 6 OCT 2008
© 2008 The Authors. Journal compilation © 2008 Japanese Society of Animal Science
Animal Science Journal
Volume 80, Issue 1, pages 70–78, February 2009
How to Cite
RASHID, H.-u., TOGO, K., UEDA, M. and MIYAMOTO, T. (2009), Characterization of bacteriocin produced by Streptococcus bovis J2 40-2 isolated from traditional fermented milk ‘Dahi’. Animal Science Journal, 80: 70–78. doi: 10.1111/j.1740-0929.2008.00596.x
- Issue published online: 21 JAN 2009
- Article first published online: 6 OCT 2008
- Received 2 August 2007; accepted for publication 30 January 2008.
- Streptococcus strain J2 40-2;
- traditional fermented milk
A bacteriocin-producing strain Streptococcus bovis J2 40-2 was isolated from traditional fermented milk ‘Dahi’ in Bangladesh. Despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as Listeria monocytogenes. Bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide pH range of 2.0–10.0. It was stable when heated to 110°C for 20 min, but lost 25% of its activity when heated to 121°C for 15 or 20 min. Optimum bacteriocin production (5600 AU/mL) was achieved when the strain was cultured at 37°C for 24 h in MRS medium rather than in TYLG, GM17, or skim milk medium. Bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut-off MW: 3500) and gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that bacteriocin had a molecular weight of approximately 4.5 kDa.