QUANTITATIVE HEPATOBILIARY SCINTIGRAPHY WITH DECONVOLUTIONAL ANALYSIS FOR THE MEASUREMETN OF HEPATIC FUNCTION IN DOGS

Authors

  • Anne Bahr DVM,

    Corresponding author
    1. Departments of Large Animal Clincical Sciences (Bahr), Small Animal Clinical Sciences (Daniel, Denovo, Young) and Pathology (Merryman), College of Veterinary Medicine. University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.
      Address correspondence to Anne Bahr, 413 Flicker Drive, Fort Collins, CO 80526
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  • Gregory B. Daniel DVM, MS,

    Corresponding author
    1. Departments of Large Animal Clincical Sciences (Bahr), Small Animal Clinical Sciences (Daniel, Denovo, Young) and Pathology (Merryman), College of Veterinary Medicine. University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.
      Address reprint requests to Gregory B Daniel DVM, MS, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, PO Box 1971, Knoxville, TN 37901-1071.
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  • Robert DeNovo DVM, MS,

    1. Departments of Large Animal Clincical Sciences (Bahr), Small Animal Clinical Sciences (Daniel, Denovo, Young) and Pathology (Merryman), College of Veterinary Medicine. University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.
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  • Karen Young BS,

    1. Departments of Large Animal Clincical Sciences (Bahr), Small Animal Clinical Sciences (Daniel, Denovo, Young) and Pathology (Merryman), College of Veterinary Medicine. University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.
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  • Joyce L. Merryman DVM, PhD

    1. Departments of Large Animal Clincical Sciences (Bahr), Small Animal Clinical Sciences (Daniel, Denovo, Young) and Pathology (Merryman), College of Veterinary Medicine. University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.
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  • This work was supported from a grant by Hills Pet Nutrition, Inc.

Address correspondence to Anne Bahr, 413 Flicker Drive, Fort Collins, CO 80526

Address reprint requests to Gregory B Daniel DVM, MS, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, PO Box 1971, Knoxville, TN 37901-1071.

Abstract

A nuclear medicine procedure that has been used for quantification of hepatocyte function in man is applied and validated in the dog. This procedure employs deconvolutional analysis of liver and heart time activity curves obtained following peripheral intravenous injection of hepatobiliary radiopharmaceutical. The deconvolutional analysis simulates a bolus injection of the radiopharmaceutical into the afferent blood supple of the liver which permits the calculation of the hepatic extraction fraction. Hepatic extraction fraction is a measure of hepatocyte function. In this report, the deconvolutional analysis via fast Fourier transformations and subsequent calculation of hepatic extraction fraction is validated by direct afferent intravascular ijection of 99mTc-mebrofenin. The hepatic extraction fraction determined via deconvolutional analysis was found to be the same as the first pass hepatic extraction fraction determined via deconvolutional analysis was found to be the same as the first pass hepatic extraction fraction determined by direct mesenteric (portal) vein injection. The same results can be obtained in a sedated animal, making the technique clinically applicable. Thus hepatic extraction fraction, obtained from a perlpheral intravenous injection of 99mTc-mebrofenin, can provide a quatitative measure of hepatocyte function from a non-invasive procedure. The quantitative ability to measure hepatocyte function has potential in many clinical and research situations.

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